《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (03): 225-227.

• 基础研究 • 上一篇    下一篇

电刺激迷走神经对感染性休克大鼠血浆肿瘤坏死因子α、一氧化氮合酶及一氧化氮的影响

谢守嫔1;李海龙2;梁永林2;王清峰3;李永盛4;明海霞2   

  1. 1.兰州市第一人民医院,甘肃兰州市 730050;2.甘肃中医学院,甘肃兰州市 730000; 3.江苏泰州职业技术学院,江苏泰州市 225300;4.甘肃省定西市第二人民医院，甘肃定西市 743000
  • 收稿日期:2008-12-15 出版日期:2009-03-01 发布日期:2009-03-01

Effects of Electrical Stimulation of Vagus Nerve on Plasma Tumor Necrosis Factor α, Nitric Oxide Synthases and Nitric Oxide in Septic Shock Rats

XIE Shou-pin, LI Hai-long, LIANG Yong-lin, et al   

  1. 1st Hospital of Lanzhou, Lanzhou 730050, Gansu, China
  • Received:2008-12-15 Published:2009-03-01 Online:2009-03-01

PDF (PC)

摘要： 目的 研究电刺激迷走神经对感染性休克大鼠血浆肿瘤坏死因子α（TNF-α）、一氧化氮合酶（NOS）及一氧化氮（NO）水平的影响。方法 成年雄性SD大鼠40只,采用盲肠结扎穿孔法(CLP)复制感染性休克模型，随机分为5组:假CLP组、CLP组、迷切组、电刺激左侧迷走神经组、电刺激右侧迷走神经组。各组动物均行颈总动脉置管连续监测平均动脉压,ELISA法检测血浆TNF-α，生化法检测血浆中NOS活性和NO水平。结果 CLP组术后平均动脉血压进行性下降,2 h时血浆TNF-α、NOS及NO水平显著升高；与CLP组比较,电刺激组动物平均动脉压下降幅度减轻,血浆TNF-α、NOS及NO水平显著降低。结论 电刺激左、右迷走神经均可能缓解CLP致感染性休克大鼠的进行性血压下降,降低血浆TNF-α、NOS及NO水平，有助于抗休克。

关键词: 感染性休克, 迷走神经, 电刺激, 肿瘤坏死因子α(TNF-α), 一氧化氮合酶（NOS）, 一氧化氮（NO）, 盲肠结扎穿孔法(CLP), 大鼠

Abstract: Objective To study the effect of electrical stimulation of vagus nerve on inflammatory response in septic shock rats. Methods SD rats were randomly divided into 5 groups: Group Ⅰ was the sham group, group Ⅱ with the cecal ligation and puncture (CLP) and the vagus nerve were isolated but not transected, group Ⅲ with bilateral cervical vagotomy following CLP, group Ⅳ with bilateral cervical vagotomy after CLP and the left vagus nerve trunks were stimulated with bipolar electrodes, group Ⅴ with bilateral cervical vagotomy after CLP and the right vagus nerve trunks were stimulated. The common carotid artery pressure was monitored, and the plasma tumor necrosis factor α (TNF-α), nitric oxide synthases (NOS) and nitric oxide (NO) were measured 2 h after stimulation. Results The mean arterial blood pressure (MAP) gradually decreased and the concentration of plasma TNF-α, NOS and NO significantly increased after CLP. Electrical stimulation of the left and right vagus nerve significantly increased the MAP and decreased the plasma TNF-α, NOS and NO levels. Conclusion Direct electrical stimulation of the left and right vagus nerve can significantly improve the blood pressure and reduced plasma TNF-α, NOS and NO levels during septic shock, which may play a role in anti-shock in rats.

Key words: septic shock, vagus nerve, electrical stimulation, tumor necrosis factor α (TNF-α), nitric oxide synthases (NOS), nitric oxide (NO), cecal ligation and puncture (CLP), rats