《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (04): 304-306.

• 专题 • 上一篇    下一篇

缺血后处理对高血脂大鼠缺血再灌注心肌细胞凋亡的影响

刘胜辉;尹博英;霍玉娥   

  1. 河北大学附属医院，河北保定市 071000
  • 收稿日期:2008-12-12 修回日期:2009-02-19 出版日期:2009-04-01 发布日期:2009-04-01

Effect of Ischemic Postconditioning on Apoptotic Myocardial Cells in Hyperlipemia Rats with Ischemic Reperfusion

LIU Sheng-hui, YIN Bo-ying, HUO Yu-e   

  1. Affiliated Hospital of Hebei University, Baoding 071000, Hebei, China
  • Received:2008-12-12 Revised:2009-02-19 Published:2009-04-01 Online:2009-04-01

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摘要： 目的 观察缺血后处理对高血脂大鼠缺血再灌注心肌细胞凋亡的影响。方法 选择高血脂SD大鼠48只，随机分为假手术组、缺血再灌注组和缺血后处理组，每组16只。制备大鼠心肌缺血再灌注模型。缺血再灌注组收紧结扎线缺血40 min，放松结扎线再灌注240 min；缺血后处理组缺血40 min后，再灌注10 s，再缺血10 s，连续3个循环，然后再灌注240 min；假手术组不收紧结扎线。再灌注结束后自右颈动脉分别采血测定血清肌酸激酶活性，用伊文氏兰-红四氮唑（TTC）染色和TUNEL法分别检测再灌注心肌坏死和凋亡程度。结果 再灌注结束后，肌酸激酶活性缺血后处理组和缺血再灌注组分别为（734.86±25.48）U/L和（967.64±28.16）U/L，高于假手术组的（274.28±16.94）U/L，（ P<0.05），缺血后处理组低于缺血再灌注组( P<0.05)；缺血后处理组心肌缺血区与左心室面积比值与缺血再灌注组无显著性差异，但心肌坏死区与缺血区比值为（24.8±6.7）%，低于缺血再灌注组的(38.2±7.1）%（ P<0.05)；假手术组未见明显细胞凋亡，缺血后处理组心肌细胞凋亡率为（12.7±2.8）%，低于缺血再灌注组的（20.9±3.7）%（ P<0.05）。结论 缺血后处理可减轻高血脂大鼠心肌缺血再灌注损伤，机制可能与减少心肌细胞凋亡有关。

关键词: 再灌注损伤, 后处理, 凋亡

Abstract: Objective To observe the effect of ischemic postconditioning on myocyte apoptosis in hyperlipemia rats with myocardial ischemic reperfusion.Methods Total forty-eight hyperlipemia SD rats were randomly divided into the sham-operation group, ischemic reperfusion group and ischemic postconditioning group with 16 rats in each group. The myocardial ischemic reperfusion model of rat was made. Rats in the ischemic reperfusion group were ligated for 40 min ischemia, and then reperfused for 240 min. Rats in the ischemic postconditioning group were treated for 40 min ischemia, reperfused for 10 s and 10 s ischemia for three cycles, and then reperfused for 240 min. Rats in the sham-operation group were opened the chest to braid for ringer without deligation. The presence of apoptotic myocytes was detected by the method of terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL). Meanwhile, the serum creatine kinase activity and myocardial infarct size were measured.Results The creatine kinase activity was 734.86±25.48 U/L in the ischemic postconditioning group and 967.64±28.16 U/L in the ischemic reperfusion group after reperfusion, all significantly higher than that (274.28±16.94 U/L) in the sham-operation group ( P<0.05), and it was significantly lower in the ischemic postconditioning group than that in the ischemic reperfusion group ( P<0.05). There was no significant difference in ratio of myocardial ischemic size and left ventricle after reperfusion between the ischemic postconditioning group and the ischemic reperfusion group. But the ratio of myocardial necrotic size and ischemic size of the ischemic postconditioning group was 24.8%±6.7% and significantly lower than that (38.2%±7.1%) of the ischemic reperfusion group ( P<0.05). No apoptotic myocardial cells were found after the reperfusion in the sham-operation group. The apoptotic rate in the ischemic postconditioning group (12.7%±2.8%) was significantly lower than that (20.9%±3.7%) in the ischemic reperfusion group ( P<0.05).Conclusion Ischemic postconditioning can relieve the ischemic reperfusion injury in hyperlipemia rats, the mechanism of which may be related with decreased myocardial apoptosis.

Key words: reperfusion injury, postconditioning, apoptosis