《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (04): 310-312.

• 专题 • 上一篇    下一篇

脂肪间充质干细胞不同移植方法治疗扩张型心肌病兔的比较

李良;夏云峰;刘润梅;翟红霞;殷亚昕;张津津   

  1. 解放军总医院第一附属医院干一科，北京市 100037
  • 收稿日期:2009-03-06 出版日期:2009-04-01 发布日期:2009-04-01
  • 通讯作者: 夏云峰

Effect of Adipose Tissue-Derived Mesenchymal Stem Cells Transplanted by Different Methods on Cardiac Function of Rabbits with Dilated Cardiomyopathy

LI Liang, XIA Yun-feng, LIU Run-mei, et al   

  1. The First Department of Cadres, the First Hospital Affiliated to the General Hospital of PLA, Beijing 100037, China
  • Received:2009-03-06 Published:2009-04-01 Online:2009-04-01

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摘要： 目的 观察脂肪间充质干细胞不同移植方法对扩张型心肌病兔心功能的影响。方法 健康白耳兔50只腹腔注射阿霉素建立扩张型心肌病模型，并随机分为模型1组、模型2组和对照组。将体外分离培养的脂肪间充质干细胞采用心肌内多点注射法移植入模型1组病兔，采用经冠状动脉移植方法植入模型2组病兔，对照组采用心肌内多点注射法注射相同体积的IMEM培养基。术后4周处死动物留取心脏，荧光显微镜下观察脂肪间充质干细胞植入体内后的存活及分化情况。于细胞移植术前和术后4周将三组兔行超声心动、血流动力学检查，测定、比较心功能指标。结果 采用两种不同移植术后，脂肪间充质干细胞均可以在病损心肌中成活，并分化为心肌细胞；移植术后4 周，模型2组与模型1组心功能比较，左心室舒张末容积及左心室收缩末容积下降( P<0.05～0.01)，左心室每搏输出量、心脏射血分数、左心室收缩压、左心室室内压最大上升速率和最大下降速率增加( P<0.05～0.01)。结论 脂肪间充质干细胞移植后可在心肌细胞内存活，并可分化为心肌细胞，改善扩张型心肌病兔的心脏功能；经冠状动脉移植较心肌内多点注射移植法更有利于发挥间充质干细胞改善心脏整体收缩功能的功效。

关键词: 脂肪间充质干细胞, 移植方法, 扩张型心肌病, 心功能

Abstract: Objective To investigate the effect of adipose tissue-derived mesenchymal stem cells (ADMSCs) transplanted by different Methods on cardiac function of rabbits with dilated cardiomyopathy.Methods 50 white ears rabbits were given doxorubicin by intraperitoneal injection to induce dilated cardiomyopathy, and randomly divided into the model group 1, model group 2 and control group. The ADMSCs isolated and cultured in vitro were transplanted into dilated cardiomyopathy rabbits of model group 1 through multi-points injection in myocardium, and were transplanted into rabbits of model group 2 through coronary artery transplant. Those in the control group were treated with IMEM medium of the same volume through multi-points injection in myocardium. Rabbits were fed for 4 week successively and then were killed to obtain heart sample, and the survival and differentiation of transplanted cells were observed through fluorescence microscope. Before transplantation and 4 weeks after transplantation, all rabbits received ultrasonic cardiogram test and haemodynamics test to determine cardiac function.Results After transplant through the two different Methods , ADMSCs could survive and differentiate in myocardial cells. 4 weeks after transplantation, the left ventricular end-diastolic volume (LVEDV) and left ventricular end-systolic volume (LVESV) reduced more significantly in the model group 2 compared with the model group 1 ( P<0.05～0.01), and LVSV, ejection fraction (EF), left ventricular systolic pressure, +dp/dtmax, -dp/dtmax increased more significantly ( P<0.05～0.01).Conclusion Transplanted ADMSCs can survive and differentiate in myocardial cells, and improve the heart function in rabbits with dilated cardiomyopathy. It is more conducive to the effectiveness of ADMSCs to improve the heart function through the method of coronary artery transplant.

Key words: adipose tissue-derived mesenchymal stem cells, method of transplant, dilated cardiomyopathy, cardiac function