《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (05): 428-430.

• 基础研究 • 上一篇    下一篇

莫诺苷抑制过氧化氢诱导的SH-SY5Y神经细胞凋亡

王文1;孙芳玲1;安宜2;艾厚喜1;张丽1;李林1   

  1. 1.首都医科大学宣武医院药物研究室，教育部神经变性病学重点实验室，北京市 100053;2.北京理工大学生命科学与技术学院生物技术系，北京市 100081
  • 收稿日期:2008-12-24 出版日期:2009-05-01 发布日期:2009-05-01
  • 通讯作者: 王文、安宜

Morroniside Inhibits Hydrogen Peroxide-induced Apoptosis in SH-SY5Y Neuroblastoma Cells

WANG Wen, SUN Fang-ling, AN Yi, et al   

  1. Department of Pharmacology, Xuanwu Hospital of Capital Medical University；Key Laboratory for Neurodegenerative Disease Ministry of Education， Beijing 100053, China
  • Received:2008-12-24 Published:2009-05-01 Online:2009-05-01

PDF (PC)

摘要： 目的 研究莫诺苷抑制过氧化氢诱导的神经细胞凋亡作用和机制。方法 培养的人神经母细胞瘤细胞系SH-SY5Y细胞加入莫诺苷1 μmol/L、10 μmol/L、100 μmol/L预孵育24 h，加入H2O2 300～500 μmol/L作用18 h，检测神经细胞丙二醛（MDA）、线粒体膜电位(MMP)、细胞凋亡。结果 1 μmol/L、10 μmol/L、100 μmol/L莫诺苷显著抑制丙二醛增加，降低细胞膜电位，抑制细胞凋亡。结论 莫诺苷能够通过抑制H2O2诱导的SH-SY5Y神经细胞脂质过氧化，降低细胞膜电位来抑制细胞凋亡。

关键词: 莫诺苷, 人神经母细胞瘤细胞系, 脂质过氧化, 线粒体膜电位(MMP), 细胞凋亡

Abstract: Objective To investigate the effects of morroniside on hydrogen peroxide (H2O2)-induced apoptosis in SH-SY5Y neuroblastoma cells. Methods SH-SY5Y cells were pre-incubated with morroniside (1, 10, and 100 μmol/L) for 24 h prior to exposure to H2O2 (300～500 μmol/L) for 18 h. The content of malondialdehyde (MDA)，mitochondrial membrane potentials (MMP) and apoptosis ratio were determined. Results Pretreatment the cells with morroniside (1, 10 and 100 μmol/L) lowered the H2O2-induced MDA concentration and MMP, and inhibited the apoptosis. Conclusion Morroniside may protect the neurocyte from H2O2-induced apoptosis by lowered MDA and inhibits MMP.

Key words: morroniside, SH-SY5Y neuroblastoma cell, lipid peroxidation, mitochondrial membrane potentials, apoptosis