《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (05): 431-433.

• 基础研究 • 上一篇    下一篇

帕金森病模型大鼠纹状体内氧化应激与细胞凋亡蛋白的表达

王涛;纪超;黎青;张清俊;左萍萍   

  1. 中国医学科学院基础医学研究所药理室，北京市 100005
  • 收稿日期:2009-02-23 出版日期:2009-05-01 发布日期:2009-05-01
  • 通讯作者: 左萍萍

Oxidative Stress and Expression of Bax and Bcl-2 in Rat Corpus Striatum Induced by 6-Hydroxydopamine

WANG Tao, JI Chao, LI Qing, et al   

  1. Department of Pharmacology, School of Basic Medicine, Peking Union Medical College & Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, China
  • Received:2009-02-23 Published:2009-05-01 Online:2009-05-01

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被引次数

22

摘要： 目的 观察帕金森病(PD)模型大鼠纹状体内的氧化应激指标及细胞凋亡相关蛋白的表达。方法 大鼠右侧纹状体内注入6-羟基多巴胺(6-OHDA)制备帕金森病模型，4周后观察行为学改变，5周后检测正常对照、假手术和模型组大鼠纹状体内超氧化物歧化酶（SOD）、谷胱苷肽过氧化物酶（GSH-Px）活性、还原型谷胱甘肽（GSH）及丙二醛（MDA）含量变化，Western方法检测Bax和Bcl-2蛋白表达。结果 在成功筛选的10只帕金森病模型大鼠右侧纹状体中，与自身对侧、正常对照及假手术组同侧相比，其SOD、GSH-Px活性，GSH含量均下降，而MDA含量升高（均P<0.05）；与正常对照及假手术组同侧相比，其Bax蛋白表达增高，而Bcl-2蛋白表达下降。结论 氧化应激在帕金森病发病机制中起到重要作用，而Bax和Bcl-2蛋白参与了氧化应激诱导细胞凋亡的调控过程。

关键词: 帕金森病(PD), 6-羟基多巴胺, 纹状体, 氧化应激, 凋亡, Bax, Bcl-2

Abstract: Objective To study the oxidative stress and apoptosis relative protein expression in rat striatum during the pathogenesis of Parkinson's disease (PD) induced by 6-hydroxydopamine (6-OHDA). Methods 6-OHDA was stereotacticly injected into the right striatum of the rats at two sites to produce PD models. Activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), contents of reduced glutathione (GSH) and malondialdehyde (MDA) in the injured and normal striatum were measured using assay kits; and levels of Bax and Bcl-2 were detected by Western blotting in injured striatum. Results In 10 successful PD rats, compared with either the sham group or the normal group, activities of SOD and GSH-Px and contents of GSH in the right striatum significantly decreased while contents of MDA increased obviously (P<0.05); And levels of Bax significantly increased while expression of Bcl-2 obviously decreased. Conclusion Oxidative stress plays a key role in the pathogenesis of PD. Furthermore, Bax and Bcl-2 were involved in the regulation of apoptosis under oxidative stress induced by 6-OHDA.

Key words: Parkinson's disease (PD), 6-hydroxydopamine, corpus striatum, oxidative stress, apoptosis, Bax, Bcl-2