《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (07): 687-690.

• 基础研究 • 上一篇    下一篇

低频超声诱导改进的PLGA微囊药物释放的体外研究

辛鹏程1a,2;王炜3;周秦武1a;吴勇杰3;王珏1b;卞正中1a   

  1. 1.西安交通大学生物医学信息工程教育部重点实验室,生命科学与技术学院,a.生物医学工程系;b.生物医学工程研究所，陕西西安市 710049; 2.兰州大学第一医院，甘肃兰州市 730000; 3.兰州大学，甘肃省新药临床前研究重点实验室，甘肃兰州市 730000
  • 收稿日期:2009-06-10 出版日期:2009-07-01 发布日期:2009-07-01
  • 通讯作者: 卞正中

Low Frequency Ultrasound Triggering Drug Release from Improved PLGA Microcapsules in Vitro

XIN Peng-cheng,WANG Wei,ZHOU Qin-wu,et al   

  1. Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Biomedical Engineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, Shaanxi, China
  • Received:2009-06-10 Published:2009-07-01 Online:2009-07-01

PDF (PC)

被引次数

15

摘要： 目的 研究低频超声对改进的PLGA微囊体外药物释放的影响，探讨以该种微囊作为将阿霉素传递到脑组织的超声靶向药物载体的可行性。方法 以水溶性药物阿霉素为模型药，用双乳化/溶剂挥发法制备PLGA微囊，并分别用壳聚糖和明胶进行包衣处理。在低频脉冲超声场（25 kHz)和连续波超声场（35.1 kHz)中对微囊进行处理，测定超声场中微囊的药物释放量。结果 壳聚糖包衣和明胶包衣都能明显降低PLGA微囊的突释效应; 明胶包衣的PLGA微囊在超声处理下药物的释放明显增加，且脉冲超声的作用要强于连续波超声。结论 明胶包衣的PLGA微囊很好的药物控释能力，其药物释放可被25 kHz脉冲超声触发，可望用作超声靶向药物进入脑组织的药物载体。

关键词: 低频超声, 药物传递, 阿霉素, 聚合物, 聚乙交酯丙交酯共聚物（PLGA）, 微囊, 明胶, 壳聚糖

Abstract: Objective To study the effect of low frequency on drug release from improved PLGA microcapsules, and investigate the possibility of utilizing PLGA microcapsules as the carrier of ultrasound targeted drug delivery system to deliver drug into brain. Methods Doxorubicin loaded poly (D,L lactic-co-glycolic acid) (PLGA) microcapsules were prepared via double emulsion solvent evaporate method and coated with either chitosan or gelatin. In vitro drug release profile and the drug release rate under the exposure of low frequency pulsed ultrasound (25 kHz) and continuous wave ultrasound (35.1 kHz) were assayed. Results The coating with chitosan or gelatin can depress the burst of drug release. The drug release rate from uncoated and chitosan-coated microcapsules did not changed with the exposure of ultrasound, and the rate of gelatin-coated microcapsules did increased. The effect of pulsed ultrasound was stronger than that of continuous ultrasound. Conclusion The drug release from gelatin-coated PLGA microcapsules can be controlled and triggered by 25 kHz pulsed ultrasound, which may be a potent carrier of targeting drugs into brain.

Key words: low frequency ultrasound, drug delivery, doxorubicin, polymer, poly (D, L lactic-co-glycolic acid) (PLGA), microcapsule, gelatin, chitosan