《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (08): 736-739.

• 基础研究 • 上一篇    下一篇

针刺对急性脑缺血再灌注大鼠脑内IL-6 mRNA表达的影响

郭壮丽;裴海涛;韩迪;李红云;王洪霞;纪晓军   

  1. 青岛大学医学院附属医院急诊神经科,山东青岛市 266003
  • 收稿日期:2009-05-05 出版日期:2009-08-01 发布日期:2009-08-01

Effect of Acupuncture on Expression of IL-6 mRNA of Acute Cerebral Ischemia Following Reperfusion in Rats

GUO Zhuang-li,PEI Hai-tao,HAN Di,et al   

  1. The Affiliated Hospital of Medical College Qingdao University, Qingdao 266003, Shandong, China
  • Received:2009-05-05 Published:2009-08-01 Online:2009-08-01

PDF (PC)

被引次数

17

摘要： 目的 观察针刺对急性脑缺血再灌注大鼠脑内IL-6 mRNA基因表达规律的影响。方法 应用大鼠脑缺血再灌注模型，采用原位杂交方法观察脑缺血再灌注后假手术组、对照组与针刺组再灌注2 h、6 h、12 h、24 h各时间点IL-6 mRNA基因表达的变化，同时应用透射电镜观察对照组及针刺组再灌注24 h后大鼠缺血区额顶叶皮质超微结构。结果 假手术组大鼠IL-6 mRNA在皮层、纹状体呈基础水平表达，对照组缺血区皮层、纹状体于缺血后2 h IL-6 mRNA表达开始升高，于24 h达高峰。且纹状体区表达较皮层强烈。阳性细胞多位于梗死区周围。除在缺血再灌注后2 h外，针刺2组在缺血区皮层、纹状体区IL-6 mRNA各时间点表达明显增强，与对照2组相比具有显著性差异(P<0.05～0.01)。针刺对缺血再灌注后细胞核的变性，细胞器的坏死有明显的改善作用，可使线粒体，内质网结构恢复正常，恢复细胞核的完整性，与对照组有明显差异。结论 针刺可明显增强皮层及纹状体IL-6 mRNA的表达。

关键词: 针刺, 脑缺血再灌注, 白细胞介素-6mRNA

Abstract: Objective To observe the expression of IL-6 mRNA and study the mechanism of acupuncture in focal cerebral ischemia.Methods In situ hybridization was used to detect the expression of IL-6 mRNA in the sham operation group, control group and acupuncture group 2 h, 6 h, 12 h, 24 h after reperfusion in the brain of middle cerebral artery occlusion rats, meanwhile and also observe ultrastructure in cortex of ischemic brain tissue 24 h after reperfusion.Results The sham operation group showed a basic expression of IL-6 mRNA in cortex and striate body. The cells expressing IL-6 mRNA were increased 2 h after ischemic reperfusion in cortex and striate body and peaked 24 h after reperfusion in control group and acupuncture group. The cells expressing IL-6 mRNA in cortex of ischemic area were stronger in acupuncture group than in control group at every research time point besides 2 h after ischemic reperfusion. The cells expressing IL-6 mRNA striate body of ischemic area were stronger in acupuncture group than in control group at every research time point. Acupuncture can lighten the degeneration of cell nucleolus and necrosis of cell, resume the integrity of the nucleolus, improve the function of mitochondrial and endoplasmic, and have significant difference with control group. Conclusion Acupuncture can increase the cells expressing of IL-6 mRNA.

Key words: acupuncture, cerebral ischemia-reperfusion, IL-6 mRNA