《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (11): 1017-1018.

• 基础研究 • 上一篇    下一篇

人骨髓间充质干细胞体外定向诱导分化为心肌样细胞的动作电位观察

邓方阁1,2;郭连峰3;马英智1;李玉林1   

  1. 1.吉林大学白求恩医学院病理生物学教育部国家重点实验室，吉林长春市 130021;2.广州医学院第一附属医院广州呼吸疾病研究所呼吸疾病国家重点实验室，广东广州市 510120;3.奥林巴斯中国有限公司生命科学部，广东广州市 510095
  • 收稿日期:2009-08-25 修回日期:2009-09-27 出版日期:2009-11-01 发布日期:2009-11-01
  • 通讯作者: 李玉林

Action Potential of Cardiomyocyte-like Cells Differentiated from Human Marrow Mesenchymal Stem Cells in Vitro

DENG Fang-ge,GUO Lian-feng,MA Ying-zhi,et al   

  1. Key Laboratory for Pathobiology of the Ministry of Education, Jilin University, Changchun 130021, Jilin, China；State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Diseases, the First Affiliated Hospital of Guangzhou Medical Co
  • Received:2009-08-25 Revised:2009-09-27 Published:2009-11-01 Online:2009-11-01

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被引次数

4

摘要： 目的 观察诱导分化为心肌样细胞的人骨髓间充质干细胞（hMSCs）是否可产生动作电位。方法 体外分离培养hMSCs，5-氮胞苷诱导向心肌细胞（CMs）分化。分别以体外分离培养的原代搏动的CMs和同代次的hMSCs为阳性对照和阴性对照，利用膜片钳技术观察动作电位。结果 在30个诱导分化成心肌样细胞的hMSCs中有6个细胞可诱发出动作电位；CMs具有典型的动作电位；未经诱导的hMSCs没有引出动作电位，5-氮胞苷诱导后细胞可以引出微弱的动作电位。结论 实验结果表明hMSCs经5-氮胞苷诱导后，在电学特性方面具有向CMs分化的潜能；与CMs具有的电生理特性相似，这些心肌样细胞具有兴奋性。

关键词: 人骨髓间充质干细胞, 心肌样细胞, 膜片钳, 动作电位, 兴奋性

Abstract: Objective To detect whether the cardiomyocyte-like cells differentiated from human marrow mesenchymal stem cells (hMSCs) could produce action potential (AP). Methods Isolated and cultured hMSCs were induced into cardiomyocyte-like cells with 5-Azacytine in vitro. They were measured for their AP by patch clamp technique, and compared with those of hMSCs of the same generation and beating cardiomyocytes (CMs) derived from 2 day-old SD rats. Results 6/30 cardiomyocyte-like cells produced AP. The CMs produced significant AP, hMSCs appeared no AP, and cardiomyocyte-like cells appeared weak AP. Conclusion The hMSCs manifested the potential to differentiate into CMs in the electrophysiology characteristics following 5-Azacytine induction.

Key words: human mesenchymal stem cells (hMSCs), cardiomyocyte-like cells, patch clamp, action potential (AP), excitability