《中国康复理论与实践》

《中国康复理论与实践》 ›› 2010, Vol. 16 ›› Issue (10): 910-912.

• 论文 • 上一篇    下一篇

金童颗粒治疗拟抽动秽语综合征模型大鼠的药理机制研究

张如意1a，张丽1a，艾厚喜1a，叶翠飞1a，孙雪静1b，李林1a   

  1. 1.首都医科大学宣武医院，a: 药物研究室、神经变性病教育部重点实验室；b: 血液科，北京市 100053。
  • 收稿日期:2010-08-18 修回日期:1900-01-01 出版日期:2010-10-25 发布日期:2010-10-25
  • 通讯作者: 李林

Pharmacological Mechanism of Jintong Capsule on Tourette Syndrome Rat Model Induced by Iminodipropionitrile

ZHANG Ru-yi, ZHANG Li, AI Hou-xi, et al   

  1. Dept. of Pharmacology, Xuanwu Hospital, Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China
  • Received:2010-08-18 Revised:1900-01-01 Published:2010-10-25 Online:2010-10-25

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被引次数

37

摘要： 目的建立拟抽动秽语综合征(TS)大鼠模型，观察临床验方金童颗粒的治疗效果并探讨其可能的作用机理。 方法IDPN腹腔注射建立大鼠拟TS模型。将SD大鼠随机分为对照组，模型组，金童颗粒小、中、大剂量组和阳性对照药氟哌啶醇组。观察动物一般行为，旷场分析实验检测动物空间认知能力，高效液相色谱法测定大鼠纹状体单胺类神经递质含量，流式细胞仪检测大鼠外周血T淋巴细胞亚群比例。 结果金童颗粒(4.5 g/kg，9 g/kg，18 g/kg)可明显减少模型动物的异常旋转圈数和仰头次数(P<0.05)，金童颗粒(9 g/kg)能明显降低模型大鼠纹状体多巴胺含量(P<0.05)，金童颗粒(18 g/kg)能明显提高模型大鼠外周血T淋巴细胞CD4/CD8比例(P<0.05)。结论金童颗粒可以明显改善TS模型大鼠行为学，其可能的作用机制为影响模型动物的多巴胺系统。同时，金童颗粒具有改善模型动物免疫功能的作用。

关键词: 抽动秽语综合征, 刻板行为, 多巴胺, 动物模型, 金童颗粒

Abstract: ObjectiveTo observe the effects of Jintong capsule on model rats of Tourette syndrome (TS) and explore its probable pharmacological mechanisms.MethodsSD rats were randomly divided into six groups: blank control, TS model, haloperidol and three Jintong capsule treated groups. Model rats were copied by intraperitoneal injection of iminodipropionitrile (IDPN). The stereotyped behaviors of model rats were recorded. Open field test was used to detect ability of space recognition of rats, high performance liquid chromatography was used to detect content of monoamines, and flow cytometry was used to detect the ratio of T lymphocyte.ResultsJintong capsule can ameliorate the stereotyped behaviors of model rats, decrease content of dopamine in striatum and increase the ratio of CD4/CD8.ConclusionJintong capsule can improve behaviors of model rats. The potential mechanism of Jintong capsule maybe: it can affect the dopaminergic system of model rats, and Jintong can enhance the immune system of model rats.

Key words: Tourette syndrome, stereotyped behaviors, dopamine, animal model, Jintong capsule