《中国康复理论与实践》

《中国康复理论与实践》 ›› 2010, Vol. 16 ›› Issue (10): 928-930.

• 论文 • 上一篇    下一篇

莫诺苷对局灶性脑缺血再灌注大鼠皮层IL-1β的影响

艾厚喜1，汪莹1,2，许栋明3，王文1，高东明2，张丽1，李林1   

  1. 1.首都医科大学宣武医院，北京市 100053；2.辽宁医学院，辽宁锦州市 121000；3.科技部火炬高技术产业开发中心，北京市 100038。
  • 收稿日期:2010-08-04 修回日期:2010-09-02 出版日期:2010-10-25 发布日期:2010-10-25
  • 通讯作者: 王文

Effects of Morroniside on IL-1β in Rat Cortex with Focal Cerebral Ischemia/Reperfusion

AI Hou-xi, WANG Ying, XU Dong-ming, et al   

  1. Torch High Technology Industry Development Center, The Ministry of Science and Technology of the P.R.C., Xuanwu Hospital of Capital Medical University, Beijing 100053, China
  • Received:2010-08-04 Revised:2010-09-02 Published:2010-10-25 Online:2010-10-25

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摘要： 目的研究山茱萸有效成分莫诺苷对局灶性脑缺血再灌注大鼠皮层IL-1β的影响。方法用线栓法制备大鼠局灶性脑缺血再灌注模型，缺血30 min，再灌注72 h。以秋水仙碱为阳性对照药，采用酶联免疫吸附法(ELISA)检测大鼠脑皮层炎症因子IL-1β的变化。结果莫诺苷可剂量依赖性地明显降低由脑缺血再灌引起的IL-1β含量增加(P<0.001)。结论莫诺苷能通过增强大鼠皮层抗炎能力发挥神经保护作用。

关键词: 莫诺苷, 缺血再灌注, IL-β, 抗炎, 神经保护

Abstract: ObjectiveTo investigate the effects of morroniside on IL-1β in rat cortex with focal cerebral ischemia/reperfusion.MethodsThe animal model was induced by occlusion of middle cerebral artery with suture embolus, ischemia for 30 min, and reperfusion for 72 h in rats. The content of IL-1β was detected with enzyme linked immunosorbent assay(ELISA).ResultsCompared with sham group, the content of IL-1β increased obviously in model rat. Compared with model group, morroniside(30 mg/kg, 90 mg/kg, 270 mg/kg) and colchicine (0.06 mg/kg) decreased the content of IL-1β significantly (P<0.001).ConclusionMorroniside may protect the cortex from inflammatory factor IL-1β.

Key words: morroniside, cerebral ischemia/reperfusion, IL-1β, antiinflammatory, neuroprotective