《中国康复理论与实践》

《中国康复理论与实践》 ›› 2010, Vol. 16 ›› Issue (10): 937-938.

• 论文 • 上一篇    下一篇

小鼠脑突触体制备方法的改进

肖忠新1，魏守刚1，云少君1，贺晓娟1，张淑华1，蔡青2，鲁强2，姬曼2   

  1. 1.首都医科大学公共卫生与家庭医学学院，北京市 100069；2.首都医科大学北京神经科学研究所，北京市 100069。
  • 收稿日期:2010-07-15 修回日期:2010-09-10 出版日期:2010-10-25 发布日期:2010-10-25
  • 通讯作者: 魏守刚

A New Rapid Method for Preparing Synaptosomes of Mouse

XIAO Zhong-xin, WEI Shou-gang, YUN Shao-jun, et al   

  1. School of Public Health and Family Medicine, Capital Medical University, Beijing 100069, China
  • Received:2010-07-15 Revised:2010-09-10 Published:2010-10-25 Online:2010-10-25

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摘要： 目的建立小鼠突触体快速制备的新实验方法。方法采用密度梯度离心法制备小鼠突触体，透射电镜观察其形态。结果本实验所获突触体具有膜包围的连续椭圆形结构，其中可见线粒体和大量突触小泡，具有典型突触体形态结构特征。结论本实验提供一种快速、简便、有效的小鼠突触体制备方法。

关键词: 突触体, 透射电镜

Abstract: ObjectiveTo establish a new method for preparing synaptosomes.MethodsDensity gradient centrifugation method was used to isolate synaptosomes of mouse, checking by transmission electron microscopy.ResultsSynaptosomes prepared by this method had intact morphological characteristics, surrounding with a continuous oval-shaped membrane structure, moreover, mitochondrion and lots of synaptic vesicle in them.ConclusionThis method is applicable to establish a rapid, convenient and useful method for preparing synaptosomes.

Key words: Synaptosomes, transmission electron microscopy