《中国康复理论与实践》

《中国康复理论与实践》 ›› 2010, Vol. 16 ›› Issue (11): 1024-1026.

• 论文 • 上一篇    下一篇

慢性心力衰竭犬心肌过表达肌浆网Ca2+-ATP酶的安全性分析

付治卿1a，李小鹰1a，鲁晓春1a，杨广1a，米亚非1a，周声安1a，叶卫华1b   

  1. 1.中国人民解放军总医院，a.南楼心血管一科;b.心外科，北京市 100853
  • 收稿日期:2010-06-11 修回日期:1900-01-01 出版日期:2010-11-25 发布日期:2010-11-25
  • 通讯作者: 李小鹰

Safety of Sarcoplasmic Reticulum Ca2+-ATPase Gene Transfer by Intramyocardium Administration in Chronic Heart Failure Beagles

FU Zhi-qing, LI Xiao-ying, LU Xiao-chun, et al   

  1. Department of Geriatric Cardiology, Chinese PLA General Hospital, Beijing 100853, China
  • Received:2010-06-11 Revised:1900-01-01 Published:2010-11-25 Online:2010-11-25

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摘要： 目的研究心肌注射法转导肌浆网Ca2+-ATP酶(SERCA2a)基因治疗慢性心力衰竭犬的安全性。方法检测SERCA2a基因转导心力衰竭犬的心肌酶学、心肌组织内cAMP含量、心肌耗氧量、心律失常及系统炎症指标、肝肾功能。结果SERCA2a基因转导治疗不增加细胞内cAMP浓度，心律失常的发生率和心肌氧耗未增加，未引起明显的全身炎症反应和肝肾功能的损伤。结论SERCA2a基因转导是一种安全的治疗策略。

关键词: 慢性心力衰竭, 肌浆网Ca2+-ATP酶, 基因治疗, 心肌注射, 安全性, 犬

Abstract: ObjectiveTo assess the safety of intramyocardium injection of sarcoplasmic reticulum Ca2+-ATPase (SERCA2a) to rescue chronic heart failure beagles. MethodsThe heart failure beagles were assessed with myocardium enzymology, cAMP content of myocardium, myocardial oxygen consumption, arrhythmia, systemic inflammation factors, and the function of liver and kidney after SERCA2a gene transferred. ResultsThere were no increase of cAMP content, no more arrhythmia, myocardial oxygen consumption, no apparente systemic inflammation, and no injures to hepatic and renal function. ConclusionOverexpression of SERCA2a by intramyocardium injection is relatively safe.

Key words: chronic heart failure, sarcoplasmic reticulum Ca2+-ATPase, gene therapy, intramyocardium injection, safety, beagles