《中国康复理论与实践》 ›› 2010, Vol. 16 ›› Issue (4): 339-341.

• 论文 • 上一篇    下一篇

大鼠全脑缺血-再灌注损伤后APC-Cdh1蛋白的表达

陈志则,万里,祁月红,姚文龙,邱瑾,张传汉   

  1. 华中科技大学同济医学院附属同济医院麻醉学教研室,湖北武汉市 430030。
  • 收稿日期:2010-01-15 修回日期:1900-01-01 出版日期:2010-04-25 发布日期:2010-04-25
  • 通讯作者: 张传汉

Expression of APC-Cdh1 Protein after Cerebral Ischemia-reperfusion Damage in Rats

CHEN Zhi-ze, WAN Li, QI Yue-hong, et al.   

  1. Department of Anesthesiology, Tongji Hospital of Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, Hubei, China
  • Received:2010-01-15 Revised:1900-01-01 Published:2010-04-25 Online:2010-04-25

摘要: 目的探讨大鼠全脑缺血-再灌注损伤后APC-Cdh1蛋白的表达变化。方法雄性SD大鼠60只,体重280~350 g,随机分成假手术组和缺血-再灌注组。采用改良Pulsinelli 4-VO法建立SD大鼠全脑缺血模型,在损伤后不同时间点,免疫组化染色检测Cdh1的表达部位并且采用Western blotting检测大鼠海马组织APC-Cdh1蛋白的表达。结果免疫组化检测显示APC-Cdh1在海马区及皮层区中均有大量表达。与假手术组相比,缺血-再灌注组术后第1天APC-Cdh1蛋白表达减少,术后第3天升高(P<0-05),术后第7天又降低。结论APC-Cdh1可能与中枢神经系统损伤有着密切的关系。

关键词: 脑缺血, 海马, 细胞周期后期分裂促进复合物

Abstract: ObjectiveTo investigate the expression of APC-Cdh1 protein after cerebral ischemia-reperfusion injury.Methods60 male Sprague-Dawley rats were randomly divided into Sham-operated group(SH) and ischemia-reperfusion group(IR). The rats of ischemia-reperfusion groups were induced by four-vessel occlusion (4-VO). At different times after injury, the expression of APC-Cdh1 of rat hippocampus was observed by Western blotting and immunohistochemistry.ResultsCompared with sham-operated group, the expression of Cdh1 protein significantly decreased 1 day and increased obviously 3 days, but decreased again 7 days after injury in ischemia-reperfusion group. The immuno-staining showed that APC-Cdh1 was highly cerebral cortex and hippocampus in ischemia-reperfusion group. ConclusionAPC-Cdh1 may be involved in the central nervous system injury.

Key words: brain ischemia, hippocampus, anaphase promoting complex