《中国康复理论与实践》

《中国康复理论与实践》 ›› 2010, Vol. 16 ›› Issue (6): 523-534.

• 论文 • 上一篇    下一篇

针刺对2型糖尿病大鼠抵抗素、脂联素及其基因表达的影响

孙志1，马丽1，韩海荣2   

  1. 1.南京中医药大学第二临床医学院，江苏南京市 210029；2.济宁医学院中医教研室，山东济宁市 272013。
  • 收稿日期:2010-03-16 修回日期:1900-01-01 出版日期:2010-06-25 发布日期:2010-06-25

Effect of Acupuncture on Resistin and Adiponectin and its Gene Expression in Type 2 Diabetic Rats

SUN Zhi, MA Li, HAN Hai-rong.   

  1. Nanjing University of TCM, Nanjing 210029, Jiangsu, China
  • Received:2010-03-16 Revised:1900-01-01 Published:2010-06-25 Online:2010-06-25

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摘要： 目的探讨针刺对2型糖尿病大鼠抵抗素、脂联素基因表达的作用。方法将造模成功的大鼠按随机化原则分为针刺组、西药组、模型组，同时将普食组大鼠作为正常对照组，每组各8只。针刺组大鼠放入固定器中，取“后三里”(相当于足三里)、内庭和胰俞穴针刺治疗，西药组用罗格列酮灌胃，模型组用双蒸水灌胃，1次/d，连续4周。实验结束后，处死动物取材，检测血清抵抗素、脂联素的含量，RT-PCR检测抵抗素、脂联素的基因表达。结果针刺组在血糖、血脂明显降低的同时，血清抵抗素明显低于模型组(P＜0.01)，脂联素高于模型组(P＜0.05)；西药组抵抗素与模型组相比明显下降(P＜0.01)，但脂联素无提高。针刺组大鼠抵抗素基因表达低于模型组和西药组，脂联素基因表达提高。结论针刺对2型糖尿病大鼠具有明显降低抵抗素基因表达和提高脂联素基因表达的作用，从而改善胰岛素抵抗。

关键词: 针刺, 2型糖尿病, 抵抗素, 脂联素, 基因表达

Abstract: ObjectiveTo study the effect of acupuncture on resistin and adiponectin and its gene expression in type 2 diabetic rats.MethodsThe rats of type 2 diabetes model were randomly divided into acupuncture group, rosiglitazone group and model control group, meanwhile 8 normal rats were assigned as the normal control group. The changes of total cholesterol(TC), triglycerides(TG), high density lipoprotein-cholesterol(HDL-C) and fasting plasma glucose (FPG) were measured. The expression of resistin gene and adiponectin gene in the adipose tissue was determined with RT-PCR. The level of serum resistin and adiponectin in type 2 diabetes rats were observed before and after acupuncture.ResultsThe levels of TC, TG, FPG resistin lowered obviously, and the levels of HDL-C adiponectin raised significantly. The expression of resistin gene lowered obviously. The expression of adiponectin gene significantly raised in acupuncture group, compared with the model control(P＜0.01, P＜0.05).ConclusionAcupuncture can decrease resistin gene expression, raise adiponectin gene expression, accordingly release insulin resistance.

Key words: acupuncture, type 2 diabetes mellitus, resistin, adiponectin, gene expression