《中国康复理论与实践》

《中国康复理论与实践》 ›› 2010, Vol. 16 ›› Issue (6): 528-531.

• 论文 • 上一篇    下一篇

环氧化酶-1在坐骨神经冷冻致神经病理性疼痛模型大鼠脊髓的表达

韩彬彬，冯艺   

  1. 北京大学人民医院麻醉科，北京市 100034。
  • 收稿日期:2010-04-28 修回日期:1900-01-01 出版日期:2010-06-25 发布日期:2010-06-25
  • 通讯作者: 冯艺

Expression of Cyclooxygenase-1 in Rat Spinal Cord of a Neuropathic Modal: Sciatic Cryoneurolysis

HAN Bin-bin, FENG Yi.   

  1. Department of Anesthesiology, Peking University People's Hospital, Beijing 100044, China
  • Received:2010-04-28 Revised:1900-01-01 Published:2010-06-25 Online:2010-06-25

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摘要： 目的观察大鼠坐骨神经冷冻(SCN)后行为学变化以及脊髓环氧化酶-1(COX-1)的表达。方法72只SD大鼠随机分为3组：正常对照组(N组，n=6)，假手术组(S组，n=33)和冷冻手术组(C组，n=33)，在制模前及制模后1、2和4周进行机械缩足阈测定和自噬评分，每时间点取6只用免疫组织化学染色的方法测定腰膨大脊髓COX-1的表达水平。结果术后冷冻组所有大鼠患侧机械缩足阈在4周时下降，低于术前及正常对照组(P<0.05)；对侧下肢机械缩足阈从术后1周开始下降，低于术前和正常对照组(P<0.05)，并持续到术后4周。自噬仅发生在冷冻组患侧后足，发生率(1周为40%，2周为43.8%)和严重程度在术后1周至2周内最高。与假手术组比较，术后2周和4周冷冻组脊髓背角COX-1表达增加(P<0.05)。结论脊髓COX-1在冷冻致神经病理性疼痛中枢敏化中有重要作用。

关键词: 环氧化酶-1, 坐骨神经冷冻, 神经病理性疼痛, 机械缩足阈

Abstract: ObjectiveTo observe behavioral changes and the dynamic expression of cyclooxygenase-1(COX-1) in the spinal cord of rats after unilateral sciatic cryoneurolysis(SCN).Methods72 male SD rats were randomly divided into 3 groups: normal control group(N, n=6), sham group(S, n=33) and cryoneurolysis group(C, n=33). The mechanical withdrawal threshold and autotomy assession were assessed before and 1, 2, 4 weeks after operation and COX-1 immunohistochemistry was performed on L4-6 spinal cord in 6 rats.ResultsFollowing SCN, rats exhibited significant bilateral tactile hypersensitivity until 4 weeks after operation. Furthermore, we observed autotomy peaked in severity and incidence (in 40% of rats, 1 weeks and 43.8%, 2 weeks ) 1 to 2 weeks after SCN. The COX-1 expression in dorsal horn of the spinal cord in group C was significantly higher than group S 2 and 4 weeks after operation (P<0.05).ConclusionCOX-1 plays an important role in spinal cord pain processing and central sensitization after SCN.

Key words: cyclooxygenase-1, sciatic cryoneurolysis, neuropathic pain, mechanical paw withdraw threshold