《中国康复理论与实践》

《中国康复理论与实践》 ›› 2010, Vol. 16 ›› Issue (8): 744-747.

• 论文 • 上一篇    下一篇

超短波对大鼠坐骨神经损伤后神经传导速度及其损伤运动神经元内VEGF表达的影响

李巍巍1,2，苑秀华2，张立新2，杨坚1   

  1. 1.上海市徐汇区中心医院康复科，上海市 200031；2.中国医科大学附属第一医院康复医学科，辽宁沈阳市 110001
  • 收稿日期:2010-05-20 修回日期:2010-06-30 出版日期:2010-08-25 发布日期:2010-08-25
  • 通讯作者: 苑秀华

Effect of Ultrashort Wave Therapy on Motor Conduction Velocity and Expression of Vascular Endothelial Growth Factor of Damaged Movement Neurons in Rats with Sciatic Nerve Injury

LI Wei-wei, YUAN Xiu-hua，ZHANG Li-xin, et al   

  1. Department of Physical Medicine and Rehabilitation, Shanghai Xuhui Center Hospital, Shanghai 200031, China
  • Received:2010-05-20 Revised:2010-06-30 Published:2010-08-25 Online:2010-08-25

PDF (PC)

摘要： 目的研究超短波治疗对大鼠坐骨神经钳夹伤后神经传导速度(MCV)及其相应水平脊髓运动神经元内血管内皮生长因子(VEGF)表达的影响。方法选取60只SD大鼠，钳夹其坐骨神经制作周围神经损伤模型，然后随机分为实验组、对照组各24只和假手术组12只。实验组术后对其坐骨神经钳夹伤处进行超短波辐射。对照组术后进行无效辐射。假手术组仅暴露单侧坐骨神经。三组大鼠分别于术后1、2、4和6周末采用电生理及免疫组织化学染色方法观察超短波治疗对大鼠坐骨神经MCV及其L4～5脊髓运动神经元VEGF阳性染色颗粒及吸光度水平的变化。结果术后1周，实验组和对照组大鼠损伤侧坐骨神经MCV值均未测出，术后2周起实验组和对照组大鼠损伤侧坐骨神经MCV值可测出，且超短波治疗组大鼠术后2、4、6周其损伤侧坐骨神经MCV值均明显高于对照组(P<0.05)。超短波治疗组大鼠L4～5脊髓运动神经元VEGF阳性染色颗粒平均积分吸光度值(IOD)在术后各同一时间点均高于对照组(P<0.05)。结论超短波能促进大鼠周围神经损伤后MCV的恢复，增加其损伤脊髓运动神经元中VEGF的表达，改善组织缺血缺氧，对大鼠坐骨神经钳夹伤后神经的修复起保护作用。

关键词: 超短波, 坐骨神经损伤, 运动神经传导速度, 血管内皮生长因子

Abstract: ObjectiveTo investigate the effect of ultrashort wave therapy on motor conduction velocity (MCV) and expression of vascular endothelial growth factor (VEGF) in rats with sciatic nerve injury.MethodsThe models of peripheral nerve injury were established by forceps clip of sciatic nerve and then a total of 60 SD rats were divided randomly into three groups, including experimental group (n=24), control group (n=24) and sham operation group(n=12). The rats of experimental group were treated by ultrashort wave therapy after operation. The injured sciatic nerve and spinal cord in waist were sampled at the 1st，2nd，4th，6th week respectively after operation and were observed by electrophysiology and immunohistochemistry.ResultsAfter operation，the MCV of sciatic nerve in both experimental group rats and control group rats is zero in 1st week. Since 2nd week，the MCV of sciatic nerve in rats of both two groups began to arise and the MCV of injuried sciatic nerve in rats of experimental group was higher than that in the control group (P<0.05). In injured spinal cord motoneuron，the Integrated Optical Density(IOD) of VEGF in experimental group was higher than that in the control group (P<0.05).ConclusionUltrashort wave therapy could increase the value of MCV of sciatic nerve and the expression of VEGF in spinal cord in rats,and so it could protect the injured peripheral nerve.

Key words: ultrashort wave, sciatic nerve injury, motor conduction velocity(MCV), vascular endothelial growth factor(VEGF)