《中国康复理论与实践》

《中国康复理论与实践》 ›› 2010, Vol. 16 ›› Issue (8): 748-751.

• 论文 • 上一篇    下一篇

利用纤维蛋白胶复合血管脱细胞基质制备全生物型小口径组织工程血管支架材料的研究

刘国锋1，何志娟2，杨大平1，徐学武1，任丽虹1, 刘莺1，李庆春1   

  1. 1.哈尔滨医科大学附属第二医院整形外科，黑龙江哈尔滨市 150086；2.哈尔滨医科大学附属第一医院妇产科，黑龙江哈尔滨市 150010
  • 收稿日期:2010-07-10 修回日期:2010-07-27 出版日期:2010-08-25 发布日期:2010-08-25
  • 通讯作者: 何志娟

Constructing a Completely Biological Hybrid Scaffold for Small-Diameter Vascular Tissue Engineering Using Fibrin and Decellularized Artery

LIU Guo-feng, HE Zhi-juan, YANG Da-ping, et al   

  1. Department of Plastic Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang, China
  • Received:2010-07-10 Revised:2010-07-27 Published:2010-08-25 Online:2010-08-25

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摘要： 目的探讨利用猪纤维蛋白胶复合犬颈总动脉脱细胞基质制备全生物型小口径组织工程血管支架材料的方法。方法将猪纤维蛋白胶均匀复合于犬颈总动脉脱细胞基质的外表面，构建全生物型小口径复合纤维蛋白胶支架材料。通过组织学染色、扫描电镜观察及生物力学测试对复合纤维蛋白胶支架材料的性质进行检测。结果组织学染色结果表明猪纤维蛋白胶均匀分布于犬颈总动脉脱细胞基质的外表面；扫描电镜结果显示复合纤维蛋白胶支架材料的外表面光滑，质地均匀。复合纤维蛋白胶支架材料、脱细胞犬颈总动脉及新鲜犬颈总动脉3组标本的极限拉伸强度和爆裂强度相似，复合纤维蛋白胶支架材料组的轴向顺应性高于脱细胞犬颈总动脉组，而与新鲜犬颈总动脉组的顺应性相似。结论猪纤维蛋白胶可以均匀复合于犬颈总动脉脱细胞基质支架材料上，能够构建出结构均匀的全生物型小口径组织工程血管的支架材料，复合纤维蛋白胶支架材料具有合适的生物力学性质，能够满足小口径组织工程血管对支架材料的要求。

关键词: 血管, 组织工程, 生物支架材料, 纤维蛋白胶, 细胞外基质, 生物力学

Abstract: ObjectiveTo prepare a completely biological hybrid scaffold for small-diameter vascular tissue engineering using porcine fibrin and decellularized canine carotid artery.MethodsPorcine fibrin was sprayed coating on the external surface of decellularized canine carotid artery to construct completely biological hybrid scaffold for small-diameter vascular tissue engineering. The completely biological hybrid scaffold was evaluated with Hematoxylin and Eosin (H&E) staining, scanning electron microscopy and biomechanics test.ResultsHistology examination revealed that the porcine fibrin was sprayed coating uniformly on the external surface of decellularized canine carotid artery. Scanning electron microscopy examination confirmed that the external surface of completely biological hybrid scaffold was smooth and uniformly. Compared with fresh canine carotid artery and decellularized artery, the biological hybrid scaffold had similar burst and breaking strength. Furthermore, compared with decellularized artery, the biological hybrid scaffold had higher compliance.ConclusionThe porcine fibrin was sprayed coating uniformly on the external surface of decellularized canine carotid artery to prepare a completely biological hybrid scaffold for small-diameter vascular tissue engineering. The biological hybrid scaffold had appropriate biomechanical properties and had potential to serve as scaffolds for small-diameter vascular tissue engineering.

Key words: vascular grafts, tissue engineering, biological scaffold, fibrin, extracellular matrix, biomechanical