《中国康复理论与实践》

《中国康复理论与实践》 ›› 2010, Vol. 16 ›› Issue (9): 801-802.

• 论文 •    下一篇

莫诺苷对局灶性脑缺血再灌注大鼠Caspase-3活化程度的影响

汪莹1，高东明1，许栋明3，王文2，艾厚喜2，张丽2，李林2   

  1. 1.辽宁医学院生理教研室，辽宁锦州市 121000；2.首都医科大学宣武医院，北京市 100053；3.科技部火炬高新技术产业开发中心，北京市 100038。
  • 收稿日期:2010-07-15 修回日期:1900-01-01 出版日期:2010-09-25 发布日期:2010-09-25
  • 通讯作者: 高东明、王文

Effects of Morroniside on Activation of Caspase-3 in Rats with Focal Cerebral Ischemia/Reperfusion

WANG Ying, GAO Dong-ming, XU Dong-ming, et al   

  1. Department of Physiology, Liaoning Medical College, Jinzhou 121000, Liaoning, China
  • Received:2010-07-15 Revised:1900-01-01 Published:2010-09-25 Online:2010-09-25

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摘要： 目的研究山茱萸有效成分莫诺苷对局灶性脑缺血再灌注大鼠海马caspase-3活化程度的影响。方法用线栓法制备大鼠局灶性脑缺血再灌注模型，缺血30 min，再灌注72 h。以维生素E(VE)为阳性对照药，采用分光光度法检测脑海马组织caspase-3活性变化。结果与假手术组相比,模型组caspase-3活性明显增加(P<0.001)；与模型组相比，莫诺苷30 mg/kg、90 mg/kg、270 mg/kg均能够降低caspase-3活性，并呈剂量依赖性(P<0.05)； VE(35 mg/kg)能显著降低caspase-3活性（P<0.001)。结论莫诺苷能够抑制大鼠脑组织caspase-3活性，通过抗凋亡发挥神经保护作用。

关键词: 莫诺苷, 缺血再灌注, caspase-3, 凋亡

Abstract: ObjectiveTo investigate the effects of morroniside on activation of caspase-3 in rats with focal cerebral ischemia/reperfusion. MethodsThe model was induced with occlusion of middle cerebral artery with suture embolus, ischemia for 30 min, and reperfusion for 72 h in rats. Vitamin E used for the positive control. The activity of caspase-3 was detected with spectrophotometry. ResultsCompared with sham group, the caspase-3 activity increased obviously in model rat. Compared with model group, Morroniside (30 mg/kg,90 mg/kg,270 mg/kg) decreased the activation of caspase-3 remarkably, which was dose-dependent (P<0.05). ConclusionMorroniside may reduce apoptosis by decreasing the activation of caspase-3 in rats.

Key words: Morroniside, ischemia/reperfusion, caspase-3, apoptosis