《中国康复理论与实践》

《中国康复理论与实践》 ›› 2010, Vol. 16 ›› Issue (9): 830-832.

• 论文 • 上一篇    下一篇

黄芪注射液对心肌细胞氧化应激性损伤的保护作用

关凤英，李红，于秀霞，杨世杰   

  1. 吉林大学白求恩医学院药理学系，吉林长春市 130021。
  • 收稿日期:2010-04-19 修回日期:2010-08-05 出版日期:2010-09-25 发布日期:2010-09-25
  • 通讯作者: 杨世杰

Effects of Astragalus Injection on Myocardial Cell Damages Due to Oxidative Stress

GUAN Feng-ying, LI Hong, YU Xiu-xia, et al   

  1. Department of Pharmacology, School of Basic Medical Sciences, Jilin University, Changchun 130021, Jilin, China
  • Received:2010-04-19 Revised:2010-08-05 Published:2010-09-25 Online:2010-09-25

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被引次数

29

摘要： 目的探讨黄芪注射液(AI)对培养的心肌细胞氧化损伤的保护作用及其机制。方法体外培养的新生Wistar大鼠心肌细胞，分为对照组、 H2O2损伤组、药物治疗组： AI低、中、高（10、30、90 g/L）剂量组及AI(90 g/L)+L-NAME(20 μg/L)组。药物预先处理心肌细胞30 min后，加入H2O2损伤心肌细胞5 h，MTT法测定心肌细胞存活力，测定乳酸脱氢酶(LDH)和一氧化氮（NO）含量的变化；激光共聚焦检测心肌细胞活性氧（ROS）的变化；流式细胞仪检测心肌细胞线粒体膜电位及细胞凋亡率的变化。结果与H2O2损伤组比较，各剂量AI可提高细胞存活力 (P＜0.05)，LDH漏出量降低(P＜0.01)，活性氧荧光强度降低(P＜0.01)，NO含量升高(P＜0.01)，线粒体膜电位升高(P＜0.05)，细胞凋亡率降低(P＜0.05)。与AI高剂量组比较，L-NAME组细胞存活力降低(P＜0.01)，LDH漏出量升高(P＜0.01)，活性氧荧光强度升高(P＜0.01)， NO含量升高(P＜0.01)，线粒体膜电位降低(P＜0.05)，细胞凋亡率升高(P＜0.05)。结论黄芪注射液可对抗H2O2 对心肌细胞的损伤，其机制与诱导NO生成、抑制活性氧生成引起的细胞凋亡有关。

关键词: 黄芪注射液, 心肌细胞, 活性氧, 一氧化氮, 凋亡, 氧化应激, 大鼠

Abstract: ObjectiveTo investigate the protective effects of Astragalus injection (AI) on hydrogen peroxide (H2O2 ) induced injury in cardiomyocytes. MethodsCultured neonatal rat cardiomyocytes were divided into: control group; H2O2 group, in which cells were treated with H2O2 0.15 mmol/L for 5 h; AI+H2O2 group, in which cells were pretreated with AI (with final concentration of 10, 30， 90 g/L) 30 min before H2O2 treatment; and AI (90 g/L)+L-NAME (20 μg/L). The cardiomyocyte viability was analysed by MTT assay, lactate dehydrogenase (LDH) activity and nitric oxide (NO) content were detected in culture media. Reactive oxygen species (ROS) were measured with laser-confocal-microscopy system. Mitochondrial memberane potential (ΔΨm) and apoptosis rate were measured with flowcytometry. ResultsCardiomyocyte viability in AI (10,30, 90 g/L) groups were higher than that in H2O2 group (P＜0.05). Compared with H2O2 group, LDH activity and ROS content in AI (90 g/L) group decreased (P＜0.01), NO content increased (P＜0.01), ΔΨm of cardiomyocytes increased (P＜0.05) and apoptosis rate decreased (P＜0.05). Compared with AI (90 g/L) group, after treatment combining with L-NAME, LDH activity and ROS content increased (P＜0.01), NO content decreased (P＜0.01), ΔΨm of cardiomyocytes decreased (P＜0.05) and apoptosis rate increased (P＜0.05). ConclusionAI can protect cardiomyocytes from H2O2 injury by increasing NO content and inhibiting cardiomyocyte apoptosis induced by ROS.

Key words: Astragalus injection, cardiomyocyte, reactive oxygen species, nitric oxide, apoptosis, oxidative stress, rat