《中国康复理论与实践》

《中国康复理论与实践》 ›› 2011, Vol. 17 ›› Issue (11): 1031-1034.

• 论文 • 上一篇    下一篇

CD14 mRNA的表达在急性脑出血肠-肝轴紊乱中的作用及星蒌承气汤的干预研究

赵海滨1，唐杰1，马立华2，王威1，王帅1，张秀静1   

  1. 1.北京中医药大学第三附属医院，北京市100029；2.北京藏医院，北京市100029。
  • 收稿日期:2011-07-22 修回日期:2011-08-12 出版日期:2011-11-25 发布日期:2011-11-25

Expression of CD14 mRNA in Intestine-liver Axis Disorder during Acute Cerebral Hemorrhage and Effects of Xinglouchengqi Decoction

ZHAO Hai-bin, TANG Jie, MA Li-hua, et al.   

  1. the 3rd Affiliated Hospital of Beijing University of Traditional Chinese Medicine, Beijing 100029, China
  • Received:2011-07-22 Revised:2011-08-12 Published:2011-11-25 Online:2011-11-25

PDF (PC)

摘要： 目的研究白细胞分化抗原14(CD14)在急性脑出血应激性肝损害内毒素信号转导通路中的作用，以及星蒌承气汤的干预作用。方法采用大脑注射Ⅶ型胶原酶法建立SD大鼠急性脑出血模型，随机分为正常组、单纯脑出血组、脑出血痰热腑实证组和星蒌承气汤组，分别分为24 h、48 h、72 h 3 个时相亚组，每个亚组8 只。实时荧光定量聚合酶链反应(PCR)观察肝、肠系膜淋巴结组织CD14 mRNA 表达，并观察肝HE 染色结果。结果脑出血急性期肝、肠系膜淋巴结组织CD14 mRNA 表达升高(P<0.05)，星蒌承气汤能明显减轻肝组织病理损伤。结论CD14在介导肠源性内毒素性肝损害中起重要作用，星蒌承气汤对脑出血急性期痰热腑实证肝脏具有一定的保护作用。

关键词: 白细胞分化抗原14, 急性脑出血, 应激性肝损害, 痰热腑实证

Abstract: Objective To investigate the influence of CD14 on lipopolysaccharide (LPS) signal transduction pathway during the hepaticstress injury in acute cerebral hemorrhage, and explore the protection mechanism of Xinglouchengqi decoction. Methods The model ofacute cerebral hemorrhage was established with injection of collagenase Ⅶ into internal capsule of cerebrum. The rats were randomly dividedinto blank control group, cerebral hemorrhage group, syndrome of phledm-heat and fu-organ excess model group and Xinglouchengqigroup. Each group was divided into 3 time sub-groups (24 h, 48 h, 72 h). The expression of CD14 mRNA in liver tissue and mesentericlymph nodes were observed by real-time quantitative polymerase chain reaction. Results The expression of CD14 mRNA was higher in model-group than blank control group (P<0.05). Xinglouchengqi decoction could reduce liver pathological injury significantly. Conclusion CD14plays an important role in endotoxin-mediated liver injury. Xinglouchengqi decoction can protect the liver in cerebral hemorrhage.

Key words: cluster of differentiation 14, acute cerebral hemorrhage, hepatic stress injury, syndrome of phledm-heat and fu-organ excess