《中国康复理论与实践》

《中国康复理论与实践》 ›› 2011, Vol. 17 ›› Issue (12): 1119-1121.

• 论文 • 上一篇    下一篇

电刺激小脑顶核对缺氧缺血新生大鼠睫状神经营养因子蛋白的影响

张丽华1,2，陶德双1,2，杨本利1，王立苹1,2，孙颖1   

  1. 1.黑龙江省小儿脑性瘫痪防治疗育中心脑瘫科，黑龙江佳木斯市 154002；2.佳木斯大学儿童神经康复实验室，黑龙江佳木斯市 154002。
  • 收稿日期:2011-07-12 修回日期:1900-01-01 出版日期:2011-12-25 发布日期:2011-12-25

Effect of Fastigial Nucleus Stimulation on Ciliary Neurotrophic Factor Protein in Newborn Rats with Hypoxic-ischemic Brain Damage

ZHANG Li-hua, TAO De-shuang, YANG Ben-li, et al.   

  1. Departmant of Cerebral Palsy, Heilongjiang Rehabilitation Center for Cerebral Palsy Children, Jiamusi 154002, Heilongjiang, China
  • Received:2011-07-12 Revised:1900-01-01 Published:2011-12-25 Online:2011-12-25

PDF (PC)

摘要： 目的探讨电刺激小脑顶核对缺氧缺血脑损伤(HIBD)新生大鼠睫状神经营养因子(CNTF)蛋白及学习记忆功能的影响。方法将大鼠随机分为假手术组、模型组和电刺激组，每组又分为7d和14d2个亚组，每个亚组6只。采用结扎左侧颈总动脉并吸入氮氧混合气体2h制作新生大鼠HIBD动物模型，电刺激组于造模后第2天开始电刺激治疗，强度25mA，频率5Hz，20min/次，每天2次。分别于干预后7d、14d用Y-型迷宫检测。HE染色法光镜下观察脑神经细胞和神经纤维的病理变化，免疫组化检测脑组织CNTF蛋白水平的表达。结果电刺激组总反应时间明显短于模型组(P<0.01)，主动回避率及正确反应率均明显高于模型组(P<0.01)，皮质及海马周围CNTF蛋白面积与积分光密度值较模型组增多(P<0.05)。结论电刺激小脑顶核可以提高脑组织CNTF蛋白水平表达，促进脑损伤大鼠学习记忆能力的恢复。

关键词: 电刺激小脑顶核, 学习记忆, 脑损伤, Y-型迷宫, 睫状神经营养因子, 缺血缺氧, 大鼠

Abstract: Objective To explore oropharyngeal swallowing disorders with videofluoroscopic swallowing study (VFSS). Methods 16 patients with dysphagia accepted VFSS with 10 ml of thin barium meal (50% w/v), thick barium meal (270% w/v), biscuit coated with thick barium meal in single swallow. Their swallowing function was observed on the lateral and anterior/posterior planes, including: symmetry of pyriform sinuses, oral transit time, presence of pharyngeal delay, pharyngeal transit time, oral and pharyngeal residue, and presence of aspiration.Results 5 patients demonstrated oral swallowing disorder. 3 patients demonstrated pharyngeal swallowing disorders, that was pharyngeal delay which caused in aspiration after swallowing. 8 patients demonstrated oropharyngeal swallowing disorders, and 3 of them presented aspiration,2 patients were silent aspirators, 1 was aspiration before and 1 after swallowing. The aspiration time could not be judged from the videofluoroscopy in the other one. For 4 patients with aspiration, 3 were severe, with more than 25% of the bolus aspirated, and 1 aspirated less than 5%. Conclusion VFSS can be helpful to plan individual rehabilitation.

Key words: fastigial nucleus stimulation, learning memory, brain injury, Y-type maze, ciliary neurotrophic factor, hypoxic-ischemic, rats