《中国康复理论与实践》

《中国康复理论与实践》 ›› 2011, Vol. 17 ›› Issue (12): 1125-1127.

• 论文 • 上一篇    下一篇

组织芯片技术在脑胶质瘤研究中的应用

崔云1a，张伟2，袁芳1b，江涛2   

  1. 1.首都医科大学北京市神经外科研究所，a.神经病理室；b. 神经病理生理室，北京市 100050；2.首都医科大学附属北京天坛医院胶质瘤治疗中心，北京市 100050。
  • 收稿日期:2010-12-10 修回日期:1900-01-01 出版日期:2011-12-25 发布日期:2011-12-25
  • 通讯作者: 袁芳

Tissue Microarray Technique in Human Brain Gliomas

CUI Yun, ZHANG Wei, YUAN Fang, et al.   

  1. Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China
  • Received:2010-12-10 Revised:1900-01-01 Published:2011-12-25 Online:2011-12-25

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摘要： 目的探讨组织芯片技术在脑胶质瘤研究中的应用价值。方法构建包括50例人脑胶质瘤标本的组织芯片，运用免疫组织化学法检测细胞增殖核抗原Ki-67的表达，并用免疫组织化学法和原位杂交法检测突变型P53蛋白及野生型p53基因的表达。结果Ki-67表达水平与肿瘤病理分级之间有关(P<0.05)。突变型P53蛋白与野生型p53mRNA之间呈显著性负相关(P<0.001)。突变型P53蛋白与Ki-67的表达水平之间存在显著性正相关(P<0.05)。结论应用组织芯片技术大规模高效检测胶质瘤临床组织样本是可行的，具有重要的实用价值和广阔的应用前景。

关键词: 胶质瘤, 组织芯片, 免疫组织化学, 原位杂交

Abstract: Objective To investigate the application of tissue microarray (TMA) technique in human brain gliomas. Methods The TMAs containing 50 glioma specimens of all pathological grades were constructed. The immunohistochemistry and in situ hybridization techniques were used to detect the expressions of Ki-67, mutant P53 protein and wild-type p53 mRNA. Results The expression of Ki-67 was significantly associated with the pathological grades (P<0.05). There was significant correlation between the expression of mutant P53 protein and wild-type p53 mRNA (P<0.001), as well as p53 mutation and Ki-67 (P<0.05). Conclusion It's feasible and valuable to utilize TMA technique in research on human brain gliomas.

Key words: glioma, tissue microarray, immunohistochemistry, in situ hybridization