《中国康复理论与实践》

《中国康复理论与实践》 ›› 2011, Vol. 17 ›› Issue (2): 141-144.

• 论文 • 上一篇    下一篇

实时荧光定量PCR微卫星分析技术检测少突胶质细胞肿瘤染色体1p、19q和10q杂合性缺失

苏江1,2，江涛3a，孙振荣3b，李桂林1a，袁芳1b   

  1. 1.北京市神经外科研究所，a.神经病理室；b. 病理生理室，北京市 100050；2.首都医科大学，北京市 100069；3.首都医科大学附属北京天坛医院，a.脑胶质瘤治疗中心；b.神经外科，北京市 100050。
  • 收稿日期:2010-12-10 修回日期:1900-01-01 出版日期:2011-02-25 发布日期:2011-02-25

Loss of Heterozygosity in Oligodendroglioma by Real-time Quantitative Polymerase Chain Reaction-based Microsatellite Analysis

SU Jiang, JIANG Tao, SUN Zhen-rong, et al.   

  1. Beijing Neurosurgical Institute, Capital University of Medical Sciences, Beijing 100050, China
  • Received:2010-12-10 Revised:1900-01-01 Published:2011-02-25 Online:2011-02-25

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摘要： 目的建立检测脑胶质瘤染色体1p、19q和10q杂合性缺失(LOH)的实验室新方法。方法采用Taqman探针法对38例少突胶质细胞肿瘤标本进行染色体1p、19q和10q LOH检测。结果38例少突胶质细胞肿瘤染色体中有25例(65.7%)发生1p LOH，26例(68.4%)发生19q LOH，5例(13.2%)发生10q LOH。结论实时荧光定量PCR微卫星分析技术快速、准确性高，可以用于脑胶质瘤标本染色体LOH的检测。

关键词: 少突胶质细胞瘤, 杂合性缺失, 聚合酶链反应

Abstract: ObjectiveTo set up the new lab examination method for 1p, 19q and 10q loss of heterozygosity(LOH) in glioma.MethodsThirty-eight cases of oligodendroglioma were enrolled into the study. Real-time quantitative polymerase chain reaction-based microsatellite analysis was performed on tumor tissues in order to study the status of chromosomes 1p, 19q and 10q.ResultsAmong the 38 cases of oligodendroglioma, 25 cases (65.7%) showed 1p LOH, 26 cases (68.4%) showed 19q LOH, while 5 cases (13.2%) showed 10q LOH.ConclusionReal-time quantitative polymerase chain reaction-based microsatellite analysis is a rapid and specific for detecting LOH in glioma tissues.

Key words: oligodendroglioma, loss of heterozygosity, polymerase chain reaction