《中国康复理论与实践》

《中国康复理论与实践》 ›› 2011, Vol. 17 ›› Issue (3): 213-218.

• 论文 • 上一篇    下一篇

大鼠脊髓损伤后胶质瘢痕形成的病理学规律

张海燕，杨朝阳，李晓光   

  1. 首都医科大学北京神经科学研究所，北京市神经再生修复研究重点实验室，教育部神经变性病学重点实验室，北京市 100069。
  • 收稿日期:2011-01-17 修回日期:1900-01-01 出版日期:2011-03-25 发布日期:2011-03-25
  • 通讯作者: 李晓光

Pathology of Glial Scar after Spinal Cord Injury

ZHANG Hai-yan，YANG Zhao-yang，LI Xiao-guang.   

  1. Neuroscience Research Institute of Capital Medical University; Key Laboratory of Beijing Neuroregeneration Repair Research; Key Laboratory of Neural Degeneration of Ministry of Education, Beijing 100069, China
  • Received:2011-01-17 Revised:1900-01-01 Published:2011-03-25 Online:2011-03-25

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摘要： 目的观察大鼠胸髓完全横断损伤后进入陈旧性脊髓损伤期的时间，并确定胶质瘢痕的时间、空间分布。方法24只成年雌性Wistar大鼠采用完全横切模型，分别于术后1 d、3 d、7 d、2周、4周、2个月、3个月、3.5个月通过组织化学和免疫组织化学方法检测损伤区囊腔、胶质纤维酸性蛋白（GFAP）和NG2表达的变化。结果囊腔大小的变化和GFAP表达水平的变化都在损伤后2个月开始减缓，并于损伤后3个月达到稳定，而NG2蛋白的表达于损伤后1个月开始趋于缓和，并于3个月达到稳定。结论脊髓损伤后于1个月开始进入陈旧期，3个月达到陈旧性损伤的稳定期。

关键词: 脊髓损伤, 星形胶质细胞, 胶质瘢痕, 囊腔, NG2

Abstract: ObjectiveTo explore the time of chronic spinal cord injury and to determine the time and spatial distribution of glial scar after completely transected rat spinal cord injury. Methods24 adult female Wistar rats were completely transected spinal cord. The responses of activated glial fibrillary acidic protein (GFAP), NG2 and the length of the cavity on the 1st, 3rd, 7th day, 2nd week, 1st, 2nd, 3rd and 3.5th month after operation were examined with histochemistry and immunohistochemistry. ResultsThe length of the cavity and the expression of GFAP begun to be stable at the 2nd month post injury, and were fully stable at 3rd month. The expression of NG2 begun to be stable at the 1st month post injury, and fully stable at 3rd month. ConclusionThe injury begun to stabilize at 1st month post injury, and fully stabilized at 3rd month.

Key words: spinal cord injury, astrocyte, glial scar, cavity, NG2