《中国康复理论与实践》

《中国康复理论与实践》 ›› 2011, Vol. 17 ›› Issue (3): 223-225.

• 论文 • 上一篇    下一篇

富含血小板血浆凝胶的超微结构

马健1,2，李放1， 任大江1，叶超群1   

  1. 1.北京军区总医院骨科,北京市 100700；2.山西医科大学第二临床医学院,山西太原市 030001。
  • 收稿日期:2011-01-12 修回日期:1900-01-01 出版日期:2011-03-25 发布日期:2011-03-25
  • 通讯作者: 李放

Microstructure of Platelet-rich Plasma Gel

MA Jian, LI Fang, REN Da-jiang, et al.   

  1. General Hospital of Beijing Military Area，Beijing 100700, China
  • Received:2011-01-12 Revised:1900-01-01 Published:2011-03-25 Online:2011-03-25

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摘要： 目的观察富含血小板血浆（PRP）凝胶的超微结构。方法采用二次离心法制备PRP，分别计数全血和PRP血小板，用酶联免疫吸附法测定全血和PRP凝胶中转移生长因子（TGF）-β1及血小板源性生长因子（PDGF）-AB的浓度；同时，对PRP凝胶行大体观察、HE染色、透射及扫描电镜观察。结果PRP中的血小板浓度达到全血的4.58倍；PRP凝胶中含有高浓度的TGF-β1、PDGF-AB；扫描电镜及透射电镜观察均显示PRP凝胶主要由大量纤维蛋白网和血小板构成。结论PRP可能是构建可注射组织工程髓核的理想支架材料。

关键词: 富含血小板血浆凝胶, 生长因子, 透射电镜, 扫描电镜, 组织工程, 髓核

Abstract: ObjectiveTo observe the microstructure and ultrastructure of platelet-rich plasma gel. MethodsPRP gel samples were obtained by two-step centrifugation. The platelets were counted before and after centrifugation. TGF-β1, PDGF-AB were measured in the PRP gel and the whole blood using the enzyme-linked immunosorbent assay. PRP gel samples were observed with macroscopic observation, HE staining, transmission (TEM) and scanning electron microscopy (SEM). ResultsThe platelet concentration of PRP was 458% of whole blood. TGF-β1, PDGF-AB were found in high concentrations in PRP gel samples. Both SEM and TEM showed that PRP gel mainly contained fibrillar material with striated band similar to fibrin filaments, and platelet. ConclusionPRP gel may be an ideal injectable scaffold material for constructing tissue engineering nucleus pulposus.

Key words: platelet-rich plasma gel, growth factors, transmission electron microscopy, scanning electron microscopy, tissue engineering, nucleus pulposus