《中国康复理论与实践》

《中国康复理论与实践》 ›› 2011, Vol. 17 ›› Issue (3): 226-228.

• 论文 • 上一篇    下一篇

胎鼠端脑神经干细胞原代单层化贴壁培养

梁军1，宋晓玉2，董建将1 ，李月珍1   

  1. 1.黑龙江省牡丹江医学院组织胚胎学教研室，黑龙江牡丹江市 157011；2. 牡丹江心血管病医院成人外科，黑龙江牡丹江市 157011。
  • 收稿日期:2010-12-20 修回日期:2011-02-25 出版日期:2011-03-25 发布日期:2011-03-25
  • 通讯作者: 董建将

Adherent Monoculture of Primary Neural Stem Cells Derived Fetal Rat Telencephalon

LIANG Jun, SONG Xiao-yu, DONG Jian-jiang, et al.   

  1. Mudanjiang Medical College, Mudanjiang 157011, Heilongjiang, China
  • Received:2010-12-20 Revised:2011-02-25 Published:2011-03-25 Online:2011-03-25

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摘要： 目的介绍利用胶原酶消化特点，简单而快速地实现神经干细胞原代单层化贴壁培养的方法。方法孕14 d的SD大鼠无菌条件下取胎鼠端脑，剪碎后用含EDTA的Ⅰ型胶原酶消化，无血清培养基培养细胞，种植于涂有多聚赖氨酸的培养板；Nestin免疫荧光鉴定细胞性质；计算细胞纯度。结果细胞Nestin免疫荧光阳性；获取的神经干细胞纯度>99%。结论本方法可以简单而快速地获得原代单层化贴壁生长的神经干细胞。

关键词: 神经干细胞, 细胞培养, 端脑, 大鼠

Abstract: ObjectiveTo introduce a simple and quick method of adherent monoculture to primary neural stem cells depending on the mainly peptic characteristic of collagenase. MethodsThe fetus rat telencephalons of a pregnant 14-day-old SD rat were isolated, and tissues were treated with collagenase type Ⅰ contained EDTA following trituration. The cells were raised in polylysine culture plates with serum-free medium, and assessed with Nestin immunofluorescence. ResultsThe Nestin positve cells were obtained, and the purity was over 99%. ConclusionThis method may simply and quickly yields the primary neural stem cells that are monolayer and adherent.

Key words: neural stem cells, cell culture, telencephalon, rats