《中国康复理论与实践》

《中国康复理论与实践》 ›› 2011, Vol. 17 ›› Issue (5): 430-432.

• 论文 • 上一篇    下一篇

同体非血管化游离骨移植中骨形成的实验研究

尹禄1，潘慧1，韩泽民1，刘宝林2   

  1. 1.武警医学院附属医院口腔颌面科，天津市 300162；2.第四军医大学口腔医学院口腔颌面外科，陕西西安市 710032。
  • 收稿日期:2011-01-12 修回日期:1900-01-01 出版日期:2011-05-25 发布日期:2011-05-25
  • 通讯作者: 韩泽民

Bone Formation during Nonvascularized Bone Graft Transplantation in Dog

YIN Lu, PAN Hui, HAN Ze-min, et al.   

  1. Department of Oral-Maxillar Region, Affiliated Hospital of Medical College of Chinese Armed Police Force, Tianjin 300162, China
  • Received:2011-01-12 Revised:1900-01-01 Published:2011-05-25 Online:2011-05-25

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摘要： 目的观察非血管化游离骨块在不同离体时间点植入体内后成骨情况。方法拔除狗前磨牙。口腔黏膜完全愈合后，截除狗长度3.0 cm的无牙下颌骨段，取不带血管和软组织的全层髂骨，分别在离体40 min、120 min 内植入下颌骨缺损区。术后4、8 周取出植骨块，观察移植骨块内细胞活力和新骨形成情况。结果移植骨同期恢复了下颌骨节段性缺损的连续性。离体40min 的游离骨块植入体内后4 周和8 周时仍为活骨块，可见到大量成活的骨细胞，并有数量不等的岛状软骨形成。离体120 min 的游离骨块植入体内后4 周和8 周时为死骨块，其中未见骨细胞成活，部分骨块中见到破骨细胞。结论非血管化游离髂骨块在较短的离体时间内同体移植可以修复狗长度3.0 cm的下颌骨节段性缺损，在修复过程中可见岛状的软骨形成。新骨形成与游离骨块的离体时间有关。

关键词: 非血管化游离骨, 移植, 软骨, 细胞活力, 狗

Abstract: Objective To observe osteocyte survival and osteogenesis after nonvascularized free bone graft transplantation for canine mandible mass defect restoration. Methods An experimental canine premolar was extracted. 1 month later, the length of 3.0 cm edentulous mandible was cut off, as the same length as that nonvascularized and soft-free tissue complete ilium block was cut off and fixed into mandible defect in 40, 120 min. The survival osteocyte and osteogenesis of the nonvascularized graft were detected 4 and 8 weeks after transplantation. Results The continuity of canine mandible segmental defect of 3.0 cm length was repaired by free nonvascularized free bone graft, live osteocytes and osteogenesis were observed in non-vascularized free bone graft at off-body 40 min group in 4-8 weeks. But the nonvascularized free bone graft off-body 120 min was a dead bone, no survival osteocytes were observed, some osteoclasts were seen in the bone graft. Conclusion The length of 3.0 cm canine mandible defect can be restored by non-vascularized free bone graft; the osteocyte survival and osteogenesis are related to their off-body time before they are fixed into recipient.

Key words: nonvascularized free bone graft, transplantation, cartilage, osteocyte survival, dog