《中国康复理论与实践》

《中国康复理论与实践》 ›› 2011, Vol. 17 ›› Issue (7): 625-627.

• 论文 • 上一篇    下一篇

针刺对抑郁症睡眠障碍大鼠行为学和海马5-羟色胺受体表达水平的影响

甄君1a，范建中2，姚晓黎3，舒晓春1b，杨艳杰1a，孔梅1b   

  1. 1.中山大学附属第五医院，a.康复医学科；b.检验科，广东珠海市 519000；2. 南方医科大学南方医院康复医学科，广东广州市 510515；3. 中山大学附属第一医院神经内科，广东广州市 510000。
  • 收稿日期:2011-03-18 修回日期:1900-01-01 出版日期:2011-07-25 发布日期:2011-07-25
  • 通讯作者: 孔梅

Effects of Acupuncture on Behaviors and Expression of 5-Hydroxytryptamine Receptor in Hippocampus in Sleep Deprivation DepressionRats

ZHEN Jun, FAN Jian-zhong, YAO Xiao-li, et al.   

  1. Department of Rehabilitation Medicine, The Fifth Affiliated Hospital of SunYat-Sen University, Zhuhai 519000, Guangdong, China
  • Received:2011-03-18 Revised:1900-01-01 Published:2011-07-25 Online:2011-07-25

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被引次数

39

摘要： 目的观察针刺治疗抑郁症睡眠障碍(SDD)的分子机制。方法将Open-Field 法评分相近的36 只Wistar 大鼠随机分为3 组：正常组、模型组和针刺组，每组12 只。除正常组外，其余各组均接受18 d 不同的应激。应激18 d 后，再进行72 h 快眼动睡眠剥夺造模。针刺组每天在应激前1 h 进行针刺，连续21 d。21 d 后，计算行为得分，取大鼠海马检测5-HT1A受体、5-HT2A受体mRNA水平。结果模型组大鼠自主活动较正常组明显减少(P<0.01)；针刺组大鼠的自主活动较模型组明显增加(P<0.01)。与正常组相比，模型组大鼠海马5-HT1A受体mRNA 表达明显下降，5-HT2A受体mRNA 表达明显上升(均P<0.01)；与模型组相比，针刺组大鼠海马5-HT1A受体mRNA表达上升、5-HT2A受体mRNA表达下降(P<0.05)。结论针刺可能通过调整5-HT 受体间不平衡而发挥治疗SDD效应。

关键词: 抑郁症睡眠障碍, 5-羟色胺, 受体, 针刺, 大鼠

Abstract: Objective To explore the molecular mechanism of acupuncture on sleep deprivation depression (SDD). Methods 36 Wistar rats with similar Open-Field score were divided into normal group, model group, and acupuncture group, 12 rats in each group. Except normal group, the other groups received different stress for 18 d, and then were deprived rapid eye sleep for 72 h. Acupuncture group received acupuncture 1 h/d before the stress for 21 d. Their behaviors were observed and the levels of 5-hydroxytryptamine 1A and 2A receptors (5-HT1AR and 5-HT2AR) mRNA in hippocampus were determined. Results There was significant decrease in the autonomic-behavior in the model group compared with the normal group (P<0.01). The autonomic-behavior in acupuncture group increased compared with the model group (P<0.01). The expression of 5-HT1AR mRNA in hippocampus of model group decreased and the 5-HT2AR mRNA increased compared with the normal group (P<0.01). The expression of 5-HT1AR mRNA increased and the 5-HT2AR mRNA decreased in the hippocampus of acupuncture group compared with model group (P<0.05). Conclusion Acupuncture is effective on SDD by re-balance the function of 5-HT receptors.

Key words: sleep deprivation depression, 5-hydroxytryptamine, receptors, acupuncture, rats