《中国康复理论与实践》

《中国康复理论与实践》 ›› 2011, Vol. 17 ›› Issue (9): 840-843.

• 论文 • 上一篇    下一篇

人脑多形性胶质瘤细胞系BT325 对5 种抗肿瘤药物敏感性的研究

韩明1,2，袁芳1,2，董丽萍1,2，师忠芳1,2，袁辉1,2，杨少华1,3,4   

  1. 1.北京市神经外科研究所，北京市 100050；2. 首都医科大学，北京市 100069；3. 首都医科大学附属北京天坛医院神经外科，北京市 100050；4.北德克萨斯大学健康科学中心药理学与神经科学系，美国德克萨斯州沃思堡市 76017。
  • 收稿日期:2011-07-14 修回日期:2011-08-02 出版日期:2011-09-25 发布日期:2011-09-25

Sensitivity of Human Glioblastoma Multiforme Cell Line BT325 to Antineoplastic Drugs

HAN Ming, YUAN Fang, DONG Li-ping, et al.   

  1. Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China
  • Received:2011-07-14 Revised:2011-08-02 Published:2011-09-25 Online:2011-09-25

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被引次数

1

摘要： 目的检测人脑多形性胶质瘤细胞系BT325 对顺铂(DDP)、替尼泊甙(VM26)、尼莫斯汀(ACNU)、替莫唑胺(TMZ)及长春新碱(VCR)5 种临床常用抗肿瘤药物的敏感性，筛选脑胶质瘤化疗敏感药物。方法用含0%、10%及20%胎牛血清的DMEM培养基培养BT325 胶质瘤细胞，分别在24 h、48 h、72 h、96 h、120 h、144 h 进行细胞计数，绘制细胞生长曲线，以确定细胞生长的最佳血清浓度；采用CCK-8 法检测不同血浆峰浓度(PPC)5 种药物对BT325 胶质瘤细胞活性的影响，计算抗肿瘤药物的抑制率及IC50。结果细胞形态观察及细胞生长曲线显示含10%胎牛血清的DMEM培养基对BT325 胶质瘤细胞生长较为适宜。细胞活性检测显示DDP 在0.625×PPC(抑制率为51.8%)及以上浓度时可抑制BT325 细胞生长，在1.25×PPC(抑制率为75.1%)及以上浓度时BT325 细胞高度敏感，且有量效关系；VM26 对BT325 细胞抑制率均在79.6%以上，为高度敏感，剂量范围广且存在量效关系；VCR各浓度对BT325 细胞抑制率为50%左右，但是与药物剂量无关；各浓度ACNU、TMZ 对BT325 细胞抑制率均在34%以下，无抑制作用。结论BT325 胶质瘤细胞对DDP、VM26 敏感，对VCR、ACNU、TMZ 不敏感。

关键词: BT325 细胞, 抗肿瘤药, 药物敏感性, CCK-8 法

Abstract: Objective To examine the sensitivity of human glioblastoma multiforme cell line BT325 to 5 antineoplastic drugs, including cisplatin (DDP), teniposide (VM26), nimustine (ACNU), temozolomide (TMZ) and vincristine (VCR). Methods BT325 cells were incubated in DMEM with 10% or 20% fetal bovine serum (FBS) or without FBS respectively. The cell numbers were counted at 24 h, 48 h, 72 h,96 h, 120 h, and 144 h, then platting and growth curve were drafted. Cell counting kite-8 was used to detect the influence of 5 drugs with different concentrations on human glioma cell line BT325. Results DDP and VM26 significantly suppressed BT325 cells(>75%) viability in a dose-dependent manner, while VCR inhibited BT325 cells (50%) growth without dose-effect relationship. In contrast, ACNU and TMZwere not effective on the viability of BT325 cells. Conclusion BT325 cells were very sensitive to chemotherapeutic drugs DDP amd VM26.

Key words: BT325 cells, antineoplastic drugs, drug sensitivity, cell-countykit-8