《中国康复理论与实践》

《中国康复理论与实践》 ›› 2012, Vol. 18 ›› Issue (10): 930-933.

• 论文 • 上一篇    下一篇

减重步行训练和督脉电针对脊髓损伤大鼠神经营养因子及生长相关蛋白-43 表达的影响

李惠兰，刘兰群，卢虎英，陈之罡，徐基民，陈洪雷   

  1. 1.首都医科大学康复医学院，北京市100068；2.中国康复研究中心北京博爱医院中医科，北京市100068。
  • 收稿日期:2012-08-27 修回日期:1900-01-01 出版日期:2012-10-25 发布日期:2012-10-25

Effect of Body Weight Support Treadmill Training or Du Channel Electroacupuncture on Expressions of Nerve Growth Factor and Growth-associated Protein-43 in Rats with Spinal Cord Transection

LI Hui-lan, LIU Lan-qun, LU Hu-ying, et al   

  1. Department of Traditional Chinese Medicine, Capital Medical University School of Rehabilitation Medicine, Beijing Bo'ai Hospital, China Rehabilitation Research Centre, Beijing 100068, China
  • Received:2012-08-27 Revised:1900-01-01 Published:2012-10-25 Online:2012-10-25

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摘要： 目的研究减重步行训练、督脉电针对横断性脊髓损伤大鼠运动功能和神经营养因子(NGF)及生长相关蛋白-43(GAP-43)的影响。方法54 只成年SD大鼠建立T10脊髓横断损伤模型，随机分为对照组、减重步行训练组和督脉电针组，每组18只，再分为8 d、15 d 和30 d 3 个亚组，每亚组6 只。对各组大鼠进行BBB评分并用免疫组织化学法测定损伤尾端组织中NGF和GAP-43 的表达。结果减重步行训练组和督脉电针组各时间点BBB 评分及NGF、GAP-43 表达水平均比对照组明显增高(P<0.01)；，督脉电针15 d 和30 d 亚组BBB评分及NGF、GAP-43 表达均明显高于减重步行训练相应亚组(P<0.01)。结论减重步行训练和督脉电针均可以促进横断性脊髓损伤大鼠的运动功能恢复和脊髓组织中NGF和GAP-43 的表达，且督脉电针疗效优于减重步行训练。

关键词: 减重步行训练, 督脉电针, 脊髓损伤, 神经营养因子, 生长相关蛋白-43

Abstract: Objective To study the effects of body weight support treadmill training (BWSTT) or Du channel electroacupuncture (DcEA) on locomotion function and expressions of nerve growth factor (NGF) and growth-associated protein-43 (GAP-43) in rats with spinal cord transection. Methods 54 adult SD rats were divided into control group (n=18), BWSTT group (n=18) and DcEA group (n=18). Each group was divided into 3 subgroups (n=6) for 8 d, 15 d and 30 d respectively. Spinal cord transection was performed at T10 level. The locomotion function was assessed with Bosso-Beattie-Bresnahan locomotor rating scale for open field (BBB scale) and the expressions of NGF and GAP-43 in spinal cord tissues to the injury caudal ends were measured with immunohistochemistry. Results The scores of BBB scales and expressions of NGF and GAP-43 were higher in BWSTT group and DcEA group than those in the control group on the 8th d,15th d and 30th d respectively (P<0.01); and were higher in DcEA group than in BWSTT group on the 15th d and 30th d respectively (P<0.01). Conclusion Both BWSTT and DcEA can improve the locomotion function in rats with spinal cord transection and increase expressions of NGF and GAP-43 in spinal cord tissues, and DcEA is more effective.

Key words: body weight support treadmill training, Du channel electroacupuncture, spinal cord injury, nerve growth factor, growth-associated protein-43