《中国康复理论与实践》

《中国康复理论与实践》 ›› 2012, Vol. 18 ›› Issue (12): 1135-1137.

• 论文 • 上一篇    下一篇

癫痫患儿不同时期脑脊液和血液中一氧化氮与氨基酸含量的变化

吴浪龙1，万福生2   

  1. 1.江西省儿童医院神经康复中心，江西南昌市330006；2.南昌大学医学院生化教研室，江西南昌市330006。
  • 收稿日期:2012-10-24 修回日期:2012-11-09 出版日期:2012-12-25 发布日期:2012-12-25

Level of Nitric Oxide and Amino Acids in Cerebrospinal Fluid and Serum of Epilepsy Children in Different Time

WU Lang-long,WAN Fu-sheng.   

  1. Jiangxi Children's Hospital, Nanchang 330006, Jiangxi, China
  • Received:2012-10-24 Revised:2012-11-09 Published:2012-12-25 Online:2012-12-25

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摘要： 目的通过对癫痫患儿不同时间点脑脊液和血液中一氧化氮(NO)代谢产物NO2-与氨基酸水平的检测，阐明NO在小儿癫痫发病中的作用及临床意义。方法分别采用硝酸还原酶法、高效液相色谱(HPLC)法，检测癫痫发作后不同时间点脑脊液和血液中NO 与氨基酸的含量。结果在惊厥发作后30 min、60 min、120 min，NO2-含量明显增高(P<0.01)，而在惊厥发作后180min，NO2-含量渐降至正常(P>0.05)。在惊厥发作后30 min，谷氨酸(Glu)升高(P<0.05)，而在惊厥发作后期，Glu 的含量明显升高(P<0.01)；甘氨酸(Gly)含量在癫痫发作各组逐渐下降(P<0.05)。结论NO是内源性致痫物质，参与惊厥的点燃过程以及癫痫后期的氧化应激性脑损害过程。癫痫发作与中枢神经系统兴奋性氨基酸与抑制性氨基酸神经递质的平衡失调密切相关。兴奋性氨基酸的毒性作用是造成脑损害的主要原因之一。

关键词: 癫痫, 一氧化氮, 谷氨酸, 甘氨酸

Abstract: Objective To detect the level of nitric oxide (NO) and amino acids in cerebrospinal fluid and serum of epilepsy children in different time. Methods The level of NO and amino acids in cerebrospinal fluid and serum of epilepsy children in different time were determined with nitrate reductase and high performance liquid chromatography (HPLC) with fluorimetric detection. Results The level of NO2 - increased 30 min, 60 min, 120 min after seizures (P<0.01), but dropped to normal 180 min after seizures (P>0.05). The level of glutamate (Glu) significantly increased in all epilepsy groups (P<0.05). The level of glycine (Gly) dropped gradually in all epilepsy groups (P<0.05).Conclusion NO may participate in the generation of seizures and the injury process to brain. The imbalance between excitatory and inhibitory amino acids may play an important role in the pathogenesis of epilepsy. The toxicity of excitatory amino acids may be the main injury to the brain.

Key words: epilepsy, nitric oxide, glutamate, glycine