《中国康复理论与实践》

《中国康复理论与实践》 ›› 2012, Vol. 18 ›› Issue (3): 215-218.

• 论文 • 上一篇    下一篇

人颞叶内侧癫痫海马组织星形胶质细胞水通道蛋白4 和内向整流性钾离子通道4.1 的再分布

徐仟1,2，孙振荣3，李桂林1,2，孙异临1,2，杨少华1,2,3，袁芳1,2   

  1. 1.首都医科大学，北京市 100069；2.北京市神经外科研究所，北京市 100050；3.首都医科大学附属北京天坛医院神经外科，北京市 100050。
  • 收稿日期:2011-12-13 修回日期:1900-01-01 出版日期:2012-03-25 发布日期:2012-03-25

Loss of Perivascular Aquaporin 4 and Inwardly Rectifying Potassium Channel 4.1 in Human Mesial Temporal Lobe Epilepsy

XU Qian, SUN Zhen-rong, LI Gui-lin, et al.   

  1. Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China
  • Received:2011-12-13 Revised:1900-01-01 Published:2012-03-25 Online:2012-03-25

PDF (PC)

被引次数

12

摘要： 目的研究人颞叶内侧癫痫海马组织星形胶质细胞水通道蛋白4(AQP4)和内向整流性钾离子通道4.1(Kir4.1) 的分布。方法对10 例颞叶内侧癫痫(MTLE)和6 例非颞叶内侧癫痫(non-MTLE)手术切除海马组织，应用光镜及透射电镜观察组织学及超微结构，免疫荧光组织化学法观察星形胶质细胞AQP4 和Kir4.1 的分布。结果MTLE 海马组织星形胶质细胞大量增生伴明显肿胀，神经元显著固缩。non-MTLE 海马组织中，AQP4 和Kir4.1 在星形胶质细胞血管周围足突(pAST-ef)分布多于其他部位，呈现极性分布特点；而在MTLE 海马组织中，AQP4 和Kir4.1 在pAST-ef 分布减少，其他部位分布增加，极性分布改变。结论海马组织星形胶质细胞上AQP4 和Kir4.1 极性分布变化可能与颞叶内侧癫痫发生相关。

关键词: 颞叶内侧癫痫, 水通道蛋白4, 内向整流性钾离子通道4.1

Abstract: Objective To investigate the distribution of aquaporin 4 (AQP4) and inwardly rectifying potassium channel 4.1 (Kir4.1) inthe astrocytes from human mesial temporal lobe epilepsy (MTLE). Methods Hippocampal specimens, including 10 cases of MTLE and 6cases of non-MTLE, were observed under optical and transmission electron microscopy. The distribution of AQP4 and Kir4.1 in astrocyteswas investigated with immunoflurescence. Results Compared with non-MTLE hippocampus, the main structural changes of MTLE includedremarkable hyperplasia astrocytes, serious swelling astrocytes and distinguished astrophy neurons. In non-MTLE hippocampus, immunoflurescencesignals of AQP4 and Kir4.1 were enriched along perivascular astrocyte end-feet domain. However, it reveals significant loss ofAQP4 and Kir4.1 in perivascular astrocyte end-feet domain in MTLE. Conclusion Loss of perivascular AQP4 and Kir4.1 in the humanMTLE may help to understand the roles of astrocyte in MTLE.

Key words: mesial temporal lobe epilepsy, aquaporin 4, inwardly rectifying potassium channel 4.1