《中国康复理论与实践》

《中国康复理论与实践》 ›› 2012, Vol. 18 ›› Issue (4): 319-323.

• 论文 • 上一篇    下一篇

任务导向性训练对局灶性脑梗死大鼠前肢运动功能及缺血区突触素和生长相关蛋白-43 表达的影响

王艳，唐强，朱路文，刘宏光，张春艳   

  1. 黑龙江中医药大学附属第二医院，黑龙江 哈尔滨市 150001。
  • 收稿日期:2012-03-12 修回日期:1900-01-01 出版日期:2012-04-25 发布日期:2012-04-25

Effects of Task-oriented Training on Forelimb Motor Function and Expression of Synaptophysin and Growth Associated Protein-43 in Rats after Focal Cerebral Ischemia

WANG Yan, TANG Qiang, ZHU Lu-wen, et al.   

  1. The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150001, Heilongjiang, China
  • Received:2012-03-12 Revised:1900-01-01 Published:2012-04-25 Online:2012-04-25

PDF (PC)

摘要： 目的观察任务导向性训练对局灶性脑缺血大鼠前肢运动功能和缺血区周围皮质突触素和生长相关蛋白(GAP)-43 表达的影响。方法采用内皮素-1(ET-1)诱导局灶性脑缺血大鼠前肢损伤模型。将大鼠随机分为假手术组、模型组、任务导向组和跑台组，每组又分为3 d、7 d、14 d、21 d 4 个亚组，每个亚组各6 只。造模后24 h 对任务导向组进行前肢抓取训练，跑台组给予跑台训练，模型组不给予任何干预。用网屏实验分析大鼠的运动功能，应用免疫组化方法观察各组不同时间点缺血灶周围皮质突触素和GAP-43 表达。结果造模后14 d 和21 d 任务导向组大鼠前肢运动功能优于模型组(P<0.01)和跑台组(P<0.05)。任务导向组缺血区周围皮质突触素平均光密度值在造模后14 d 和21 d 优于模型组和跑台组(P<0.05)；任务导向组缺血区周围皮质GAP-43 阳性细胞数量在造模后7 d 和14 d 优于模型组和跑台组(P<0.05)。结论任务导向性训练法可促进局灶性脑缺血大鼠前肢运动功能的恢复，疗效优于动物跑台训练，其机制可能是与增强脑缺血区周围皮质突触素和GAP-43 的表达有关。

关键词: 任务导向性训练, 脑缺血, 前肢运动功能, 突触素, 生长相关蛋白-43, 跑台训练, 大鼠

Abstract: Objective To explore the effects of task-oriented training on forelimb motor function and expression of synaptophysin andgrowth associated protein (GAP)-43 in rats after focal cerebral ischemia. Methods Endothelin-1 (ET-1) was used to induce focal cerebralischemia model in rats with forelimb dysfunction. The rats were randomly divided into 4 groups: sham operation group, model group,task-oriented training group and treadmill running group, each group was divided into 4 subgroups by 3 d, 7 d, 14 d, and 21 d. 24 hours aftermodeling, the rats in task-oriented training group were trained with forelimb reaching and the rats in the treadmill running group weretrained by treadmill, and the model group had no intervention. Motor function of rats was evaluated by net screen test, and immunohistochemistrystaining was used to observed the expression of GAP-43 and synaptophysin in cortical ischemia lesions of the rats in each groupat different time points. Results The scores of behavioral tests were better in the task-oriented training group than in the model group (P<0.01) and the treadmill group (P<0.05) on the 14th and 21st days after modeling. The expression of GAP-43 was higher in the task-orientedtraining group than in the model group (P<0.05) and the sham group (P<0.01) on the 7th and 21st days after modeling. The expression ofsynaptophysin was higher in the task-oriented training group than in the model group (P<0.05) and the sham group (P<0.05) on the 14th andthe 21st days after modeling. Conclusion Task-oriented training can facilitate the recovery of forelimb motor function of rats after focal cerebralischemia, and it may be partially related to the up-regulation of expression of GAP-43 and synaptophysin in peri-infarction cortex.

Key words: task-oriented training, cerebral ischemia, forelimb motor function, synaptophysin, growth associated protein-43, treadmill running, rats