《中国康复理论与实践》

《中国康复理论与实践》 ›› 2012, Vol. 18 ›› Issue (4): 333-336.

• 论文 • 上一篇    下一篇

芬太尼对慢性吗啡耐受大鼠蓝斑核δ受体与β-arrestin 1 的作用

刘若杉，孙莉   

  1. 中国医学科学院肿瘤医院麻醉科，北京市 100021。
  • 收稿日期:2011-11-25 修回日期:1900-01-01 出版日期:2012-04-25 发布日期:2012-04-25

Influence of Fentanyl on Expression of δ Receptor and β-arrestin 1 in Locus Ceruleus of Chronic Morphine Tolerant Rats

LIU Ruo-shan, SUN Li.   

  1. Department of Anesthesiology, Cancer Hospital &Institute, Chinese Academy of Medical Sciences, Beijing 100021, China
  • Received:2011-11-25 Revised:1900-01-01 Published:2012-04-25 Online:2012-04-25

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摘要： 目的研究芬太尼对慢性吗啡耐受大鼠蓝斑核δ受体与β-arrestin 1 的作用。方法40 只成年雄性SD 大鼠随机分为5组，每组8 只。NS 组：注射生理盐水1 ml/kg 2 次；M组：分别注射吗啡10 mg/kg 与生理盐水1 ml/kg；MF1、MF2、MF3组：吗啡用药同M组，30 min 后分别给予芬太尼3、6、12 μg/kg，连续9 d。取大鼠蓝斑核，测定δ受体、β-arrestin 1 的mRNA与蛋白表达。结果与NS 组比较，M组δ受体、β-arrestin 1 表达明显下调(P<0.01)；与M组比较，MF1、MF2和MF3组δ受体表达无显著性差异(P>0.05)，但MF2组和MF3组β-arrestin 1 mRNA及其蛋白的表达增加(P<0.05)。结论芬太尼6 μg/kg、12 μg/kg 可上调慢性吗啡耐受大鼠蓝斑核β-arrestin 1 表达，但对δ受体表达无影响。

关键词: 吗啡耐受, 芬太尼, 蓝斑核, δ受体, β-arrestin 1

Abstract: Objective To explore the effects of fentanyl on expression of δ receptor and β-arrestin 1 in locus ceruleus of chronic morphinetolerant rats. Methods 40 male SD rats were randomly allocated to 5 groups with 8 cases in each group. Group NS received only normalsaline 1 ml/kg twice; Group M received morphine 10 mg/kg followed by normal saline 1 ml/kg; Groups MF1, MF2 and MF3 receivedmorphine 10 mg/kg followed by fentanyl 3, 6, 12 μg/kg respectively. All animals were killed on the 9th day after measurement of painthreshold. Locus ceruleus was removed for determination of the expression of mRNA (RT-PCR) and protein (Western blotting) of δ receptorand β-arrestin 1. Results The expression of δ receptor and β-arrestin 1 was significantly decreased in Group M than in Group NS (P<0.01).There was no significant difference in the expression of δ receptor mRNA and protein in Groups MF1, MF2 and MF3, and Group M (P>0.05).The expression of β-arrestin 1 mRNA and protein in Groups MF2 and MF3 significantly increased (P<0.05). Conclusion Fentayl with thedose of 6 and 12 μg/kg can dose-dependently increase the expression of β-arrestin 1 but δ receptor in locus ceruleus of chronic morphine tolerantrats.

Key words: morphine tolerance, fentanyl, locus ceruleus, delta-receptor, β-arrestin 1