《中国康复理论与实践》

《中国康复理论与实践》 ›› 2012, Vol. 18 ›› Issue (6): 535-538.

• 论文 • 上一篇    下一篇

应用人工神经修复大鼠坐骨神经损伤的实验研究

王晓亮，杨朝阳，李晓光，张皑峰   

  1. 1.首都医科大学北京神经科学研究所，北京市神经再生修复研究重点实验室，教育部神经变性病学重点实验室，北京市100069；2. 北京市友谊医院口腔科，北京市100050。
  • 收稿日期:2012-02-20 修回日期:2012-03-29 出版日期:2012-06-25 发布日期:2012-06-25

Experimental Study on Sciatic Nerve Injury Repairing by Artificial Nerve Graft in Rats

WANG Xiao-liang, YANG Zhao-yang, LI Xiao-guang, et al.   

  1. Neuroscience Research Institute of Capital Medical University, Key Laboratory of Beijing Neuroregeneration Repair Research,Key Laboratory of Neural Degeneration of Ministry of Education, Beijing 100069, China
  • Received:2012-02-20 Revised:2012-03-29 Published:2012-06-25 Online:2012-06-25

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被引次数

22

摘要： 目的观察应用复合碱性成纤维细胞生长因子(bFGF)-壳聚糖导管修复大鼠周围神经损伤的效果。方法成年Wistar大鼠25 只分为假手术组(n=10)、单纯损伤组(n=5)和人工神经修复组(n=10)。假手术组仅暴露坐骨神经5 mm，单纯损伤组暴露坐骨神经并切断，人工神经修复组以复合bFGF-壳聚糖导管桥接缺损。术后对实验动物的坐骨神经进行大体观察，对运动进行行为观察，以及组织化学和免疫组织化学方法评价神经再生情况和靶肌肉恢复情况。结果术后5 周，与单纯损伤组相比，人工神经修复组运动功能有一定程度恢复。人工神经修复组再生的坐骨神经已经通过bFGF-壳聚糖导管越过缺损并与远端相连接。免疫组织化学方法显示，坐骨神经再生段可观察到神经微丝(NF)阳性和S-100 阳性纤维。应用Masson 染色方法，可观察到人工神经修复组腓肠肌去纤维化程度相对于单纯损伤组有明显改善。结论应用bFGF-壳聚糖导管能有效修复周围神经损伤并使大鼠运动功能得到改善。

关键词: 周围神经损伤, 人工神经移植, 神经再生, 碱性成纤维细胞生长因子, 壳聚糖, 功能恢复

Abstract: Objective To observe the effect of the chitosan tube combined with basic fibroblast growth factor (bFGF) on repairing sciaticnerve injury in rats. Methods 25 adult Wistar rats were divided into modeled sham-operated group (n=10), lesion control group (n=5) andexperiment group (n=10). All of them were operated with a 5-mm sciatic nerve exposed on the left. The lesion control group was intervenedwith the sciatic nerve cut off. The experiment group was intervened with bFGF-chitosan tubes bridged into nerve stumps. The movement behaviorwas observed, the regeneration of the injury nerves and the distal target muscle were detected by morphological staining. Results 5weeks after operation, the movement function of the experiment group was apparently recovered and gaps on the sciatic nerve were connectedby the regeneration nerve in this group and NF and S-100 immunoreactive fibers were observed in regeneration nerve. Masson's trichromestain showed that the target muscle was close to the level of sham-operated group in morphology and density. Conclusion The chitosantube combined with bFGF can repair the peripheral nerve injury effectively and improve the movement function of rats.

Key words: peripheral nerve injury, artificial nerve graft, nerve regeneration, basic fibroblast growth factor, chitosan, function recovery