《中国康复理论与实践》

《中国康复理论与实践》 ›› 2012, Vol. 18 ›› Issue (6): 548-550.

• 论文 • 上一篇    下一篇

脊髓损伤后大鼠行为学改变及早期腓肠肌的变化

宋伟1,2，蒲放3   

  1. 1.首都医科大学康复医学院，北京市100068；2.中国康复研究中心康复工程研究所，北京市100068；3.北京航空航天大学生物医学工程学院，北京市100191。
  • 收稿日期:2011-08-04 修回日期:1900-01-01 出版日期:2012-06-25 发布日期:2012-06-25

Change of Ethology and Gastronomies of Rats after Spinal Cord Injury

SONG Wei, PU Fang.   

  1. Capital Medical University School of Rehabilitaion Medicine Chia Rehabiltation Research Cenrer.Beijing 100068 China
  • Received:2011-08-04 Revised:1900-01-01 Published:2012-06-25 Online:2012-06-25

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摘要： 目的探索脊髓损伤后大鼠行为学改变及早期腓肠肌变化。方法108 只Wistar 大鼠，其中38 只为假手术组(n=38)，其余制作脊髓切除模型，分为损伤组(n=38)和康复训练组(n=32)。应用BBB 法评价大鼠脊髓损伤后不同时间点的行为学变化；通过免疫组织化学法观察腓肠肌的变化。结果康复训练组BBB 评分从术后3 周开始高于损伤组，但两组得分始终未超过10 分。Dystrophin 免疫荧光染色显示，脊髓损伤后损伤组和康复训练组腓肠肌肌纤维横截面积均减小，但康复训练组萎缩程度较轻。结论脊髓损伤后大鼠后肢运动功能可出现一定的自发性恢复，康复训练有利于脊髓损伤大鼠运动功能的恢复，并能减缓后肢肌肉萎缩。

关键词: 脊髓损伤, 康复训练, 肌肉

Abstract: Objective To explore the alteration in ethology and change of muscle fibers of gastronomies of rats following spinal cord injury(SCI). Methods 108 Wistar rats were divided into sham operation group (n=38) and SCI group (n=38) and rehabilitation group (n=32).The ethology was evaluated by Basso, Beattie, and Bresnahan Locomotor Rating Scale (BBB Scale), and the alteration of muscle tissue's reactivitywas observed by immunohistochemistry staining. Results The BBB scores of rehabilitation group were higher than SCI group 3weeks after injury, but all was lower than 10. The cross section areas of gastrocnemius in both groups were getting smaller, but the rehabilitationgroup was slighter. Conclusion Spontaneous recovery occurred in some degree for rats' hind limb after spinal cord injury. Rehabilitationtraining is beneficial for the recovery of rats' motor function, and alleviates the atrophy of hind limb after spinal cord injury.

Key words: spinal cord injury, rehabilitation training, muscle