《中国康复理论与实践》

《中国康复理论与实践》 ›› 2012, Vol. 18 ›› Issue (9): 831-833.

• 论文 • 上一篇    下一篇

原花青素对脊髓损伤大鼠运动功能的影响

谢少华，杨拯，龚都，张尧，余婷，高建华，杨海燕，李晓，马延仃，张晓   

  1. 成都医学院，四川成都市610081
  • 收稿日期:2012-03-02 修回日期:1900-01-01 出版日期:2012-09-25 发布日期:2012-09-25
  • 通讯作者: 杨拯

Effect of Proanthocyanidin on Motor after Spinal Cord Injury in Rats

XIE Shao-hua, YANG Zheng, GONG Du, et al   

  1. Chengdu Medical College, Chengdu 610081, Sichuan, China
  • Received:2012-03-02 Revised:1900-01-01 Published:2012-09-25 Online:2012-09-25

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摘要： 目的观察原花青素对脊髓损伤大鼠运动功能恢复的影响。方法健康成年SD大鼠36 只，采用Allen 法(250 g·mm)在T9复制急性脊髓损伤模型，分为A、B、C 3 组，每组12 只。造模型成功后30 min，A组腹腔注射原花青素40 mg/kg，B 组腹腔注射甲基强的松龙30 mg/kg，C 组腹腔注射等体积生理盐水。于术后1 d、3 d、7 d 对各组进行后肢功能BBB 评分、斜板试验，血清丙二醛(MDA)和超氧化物歧化酶(SOD)测定。结果BBB 评分和斜板试验结果均显示，术后3 d 和7 d，A、B 两组较C 组后肢运动功能恢复更好(P<0.05)。与C 组相比，A、B 两组术后1 d 和3 d SOD 活性升高，MDA 含量降低(均P<0.05)；7 d 时，A 组较C 组SOD活性增高，MDA降低(P<0.05)。结论原花青素可有效抑制脂质过氧化反应，对脊髓损伤大鼠运动功能的恢复具有促进作用。

关键词: 原花青素, 脊髓损伤, 运动功能, 超氧化物歧化酶, 丙二醛

Abstract: Objective To observe the effect of Proanthocyanidin on motor after spinal cord injury (SCI) in rats. Methods 36 healthy adult SD rats were divided into groups A, B and C (n=12), and SCI was induced with Allen's mode (250 g·mm) on T9. Proanthocyanidin 40 mg/kg was injected intraperitoneally for group A, methylprednisolone (MP) 30 mg/kg for group B and the same volume of saline for group C 30 min after SCI. 1 d, 3 d and 7 d after operation, all the rats were assessed with Basso-Beattie-Bresnahan (BBB) scale and slanting board test, and their serumal malondialdehyde (MDA) and superoxide dismutase (SOD) were detected. Results The scores of BBB and the slanting board test imporoved more in group A and group B than in group C (P<0.05). The SOD increased and MDA decreased in groups A and B significantly 1 d and 3 d after operation compared with those of group C (P<0.05), and only in group A 7 d after operation (P<0.05). Conclusion Proanthocyanidin may inhibit the lipid peroxidation and promote the recovery of motor after spinal cord injury in rats.

Key words: Proanthocyanidin, spinal cord injury, motor, superoxide dismutase, malondialdehyde