《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (06): 510-512.

• 论文 • 上一篇    下一篇

结核分枝杆菌与两种人工关节介面黏附特点对比研究

包二平，黄迅悟，李洪敏，冯会成，董志明   

  1. 1.河北北方学院，河北张家口市075000；2.解放军第309 医院，北京市100091。
  • 收稿日期:2013-03-14 修回日期:1900-01-01 出版日期:2013-06-25 发布日期:2013-06-25
  • 通讯作者: 黄迅悟

Adherence Condition of Mycobacterium Tuberculosis on 2 Kinds of Artificial Joint Interface

BAO Er-ping, HUANG Xun-wu, LI Hong-min, et al.   

  1. Department of Orthopedics, Hebei North University, Zhangjiakou 075000, Hebei, China
  • Received:2013-03-14 Revised:1900-01-01 Published:2013-06-25 Online:2013-06-25

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摘要： 目的探讨结核分枝杆菌黏附性。方法将表面多孔涂层和羟基磷灰石涂层实验材料每组各取20 片，将每片实验材料在无菌条件下悬吊于M960 培养基中，与结核分枝杆菌共同培养。每个M960 培养基培养28 d。用721 分光光度计(波长590 nm、光镜1.0 cm、蒸馏水调零)测定培养基菌液的光密度(OD)值。用扫描电镜观察结核分枝杆菌与两种介面的黏附情况。结果两种材料的OD值无显著性差异(P>0.05)。结核分枝杆菌在多孔介面黏附量高于羟基磷灰石涂层介面(P<0.05)。结论在为关节结核患者行人工关节置换时，选择羟基磷灰石涂层比多孔涂层更有优势。

关键词: 关节, 结核, 结核分枝杆菌, 细菌黏附, 结核感染

Abstract: Objective To compare the adherence condition of mycobacterium tuberculosis on 2 kinds of artificial joint interface. Methods The Chromium-Cobalt-Alloy with hydroxyapatite ceramic coating and porous coating were cocultured with the bacterial liquid of mycobacterium tuberculosis. The optical density of mycobacterium tuberculosis in different interfaces were detected by turbidimetry. Adherence conditions were observed by electron microscope scanning. Results There was no significant difference between two groups in the optical density value (P>0.05). The electron microscope scanning showed that more mycobacterium tuberculosis adhered to the porous coating than hydroxyapatite ceramic coating (P<0.05). Conclusion The hydroxyapatite ceramic coating has more advantages to the porous coating in prosthetic replacement for patients with joint tuberculosis.

Key words: joint, tuberculosis, mycobacterium tuberculosis, bacterial adhesion, tuberculosis infection