《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (06): 561-564.

• 论文 • 上一篇    下一篇

HLA-DRB1基因多态性与糖尿病并发肺结核易感性的研究

王育华，王玉浔，安雅臣，纪春梅，刘国荣，赵晓晶，高素玲，张江，王艳，冯福民   

  1. 1.唐山市结核病医院，河北唐山市063000；2.河北联合大学，河北唐山市063000；3.唐山市协和医院，河北唐山市063000。
  • 收稿日期:2013-01-04 修回日期:2013-03-20 出版日期:2013-06-25 发布日期:2013-06-25
  • 通讯作者: 安雅臣

Relationship between HLA-DRB1 Gene Polymorphisms and Susceptibility of Pulmonary Tuberculosis in Patients with Type 2 Diabetes Mellitus: 124 Case-control Study

WANG Yu-hua, WANG Yu-xun, AN Ya-chen, et al.   

  1. Department of Neurology, Affiliated Hospital, Hebei United University, Tangshan 063000, Hebei, China
  • Received:2013-01-04 Revised:2013-03-20 Published:2013-06-25 Online:2013-06-25

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被引次数

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摘要： 目的探讨HLA-DRB1 基因多态性与我国北方汉族糖尿病并发肺结核易感性的关系。方法采用1∶1 配对的病例对照研究设计，以我国北方汉族124 例糖尿病并发肺结核患者为研究对象，以与病例同民族、同性别、年龄相差不超过3 岁，相同居住地(城市或农村)，且经统一结核菌素试验(PPD)确认为有结核分支杆菌感染的2 型糖尿病患者为对照组；采用聚合酶链反应-特异性序列引物(PCR-SSP)的方法检测HLA-DRB1 基因DR15、DR16、DR1、DR11 位点多态性，对与肺结核相关的危险因素进行问卷调查，应用SPSS软件进行单因素和多因素条件Logistic 回归分析。结果在病例组中，携带DR15 基因突变型患肺结核的风险增高(OR＝2.461, 95%CI: 1.363~4.444, P=0.002)；DR15 位点突变与DR16 位点突变同时存在时风险更大(OR=4.904, 95%CI: 1.554~15.476)。对17 个环境危险因素进行单因素分析，在多因素分析中调整卡痕、体质量指数、家族史3 个因素后，DR15 突变基因型仍与糖尿病并发肺结核显著相关(OR=2.996, 95%CI: 1.51~5.945)。结论HLA-DR15 基因突变型可能为2 型糖尿病并发肺结核的易感基因型。

关键词: 结核, 肺, 糖尿病, HLA基因, 基因多态性, 病例对照研究

Abstract: Objective To investigate the association between the HLA-DRB1 gene polymorphisms and susceptibility of pulmonary tuberculosis (TB) in patients with type 2 diabetes mellitus in North China. Methods A 1∶1 matched case-control study was adopted. Polymerase chain reaction-sequence-specific primers techniques (PCR-SSP) and restriction fragment length polymorphism (RFLP) was used to type polymorphisms (DR15, DR16, DR1, DR11). Information on environmental-related risk factors and pathological changes of tuberculosis was collected using a pre-tested standard questionnaire. Variance analysis, Chi-square, univariate and multivariate conditional Logistic analysis were conducted with SPSS 12.0 for Window. Results A sample of 124 pairs of cases and controls was studied. Univariate analysis demonstrated that DR15 mutant increased the risk of susceptibility of pulmonary tuberculosis (OR＝2.461, 95%CI: 1.363~4.444, P=0.002),and it would further increased if DR16 mutant occurred together (OR=4.904, 95%CI: 1.554~15.476). In multivariate analysis, DR15 mutant also associated with susceptibility of pulmonary tuberculosis (OR=2.996, 95%CI: 1.51~5.945). Conclusion Polymorphism of DR15 genotype might be a susceptible genotype of TB patients with type 2 diabetes mellitus in North China.

Key words: tuberculosis, pulmonary, diabetes, HLA gene, polymorphism, case-control study