《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (09): 824-827.

• 论文 • 上一篇    下一篇

发光二极管红光对实验性高脂血症大鼠血脂的调节作用

卢建丽，刘翠霞，尹昱，安经克，贾子善   

  1. 河北省人民医院康复科，河北石家庄市050051。
  • 收稿日期:2013-01-09 修回日期:2013-03-15 出版日期:2013-09-25 发布日期:2013-09-25
  • 通讯作者: 贾子善

Effect of Light Emitting Diode Red Light Irradiation on Hyperlipidemia Rats

LU Jian-li, LIU Cui-xia, YIN Yu, et al.   

  1. Department of Rehabilitation, Hebei General Hospital, Shijiazhuang 050051, Hebei, China
  • Received:2013-01-09 Revised:2013-03-15 Published:2013-09-25 Online:2013-09-25

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被引次数

6

摘要： 目的探讨发光二极管(LED)红光照射对高脂血症大鼠血脂的调节作用。方法健康雄性Sprague-Dawley 大鼠36 只随机分为正常对照组(n=12)和高脂模型组(n=24)。正常对照组饲喂普通饲料，高脂模型组采用高脂饲料喂养6 周。造模成功后，再将高脂模型组随机分为高脂对照组(n=12)和LED治疗组(n=12)。高脂对照组不给予照射，LED治疗组给予LED红光照射，波长(630±15) nm，每天30 min，连续照射28 d。生化法检测大鼠血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)水平及脂蛋白酯酶(LPL)和肝脂酶(HL)活性。肝脏组织免疫组化染色观察羟甲基戊二酰辅酶A 还原酶(HMG-CR)的表达。结果LED治疗组大鼠血清TC、TG和LDL-C水平较高脂对照组明显减低(P<0.01)，而HDL-C水平明显升高(P<0.01)；LED治疗组血清LPL 和HL 的活性明显高于高脂对照组(P<0.01)，LED 治疗组肝脏组织中HMG-CR 的表达低于高脂对照组(P<0.05)。结论LED红光照射通过增强LPL、HL 的活性及降低HMG-CR的表达，影响血清脂质的代谢，从而达到调节血脂的作用。

关键词: 红光照射, 高脂血症, 脂蛋白酯酶, 肝脂酶, 羟甲基戊二酰辅酶A还原酶, 大鼠

Abstract: Objective To observe the effect of light emitting diode (LED) red light irradiation on serum lipid in experimental hyperlipidemia rats. Methods 36 healthy male Sprague-Dawley rats were randomly divided into normal control group (n=12) and hyperlipidemic model group (n=24). The normal group was fed with normal diet while the hyperlipidemic model group with fat-rich forage for 6 weeks. The hyperlipidemic model group rats were randomly divided into the hyperlipidemic control group (n=12) and LED treatment group (n=12), and the latter accepted LED red light irradiation for 28 d. The levels of serum lipid including total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL-C), high-density lipoprotein (HDL-C), and the activities of lipoproteinesterase (LPL) and hepatic lipase (HL) were detected with biochemical assay. The expression of 3-hydroxy-3-methylglutaryl coenzyme A reducase (HMG-CR) of hepatic tissue were measured with immunohistochemical staining. Results Compared with the hyperlipidemic control group, the levels of serum TC, TG and LDL-C decreased while the serum HDL-C increased significantly in the LED treatment group (P<0.01) after treated with LED. The levels of LPL and HL in serum increased (P<0.01) while the activity of HMG-CR decreased (P<0.05). Conclusion LED red light irradiation might play a regulating effect on serum lipid by enhancing the activities of LPL and HL and inhibiting the expression of HMG-CR to interfere the metabolism of TC, TG, LDL-C and HDL-C.

Key words: red light irradiation, hyperlipidemia, lipoproteinesterase, hepatic lipase, 3-hydroxy -3-methylglutaryl coenzyme A reducase, rats