《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (10): 927-930.

• 论文 • 上一篇    下一篇

石杉碱甲干预对重复电痉挛刺激大鼠空间记忆的影响

成敬，肖玲，王高华，李承晏   

  1. 1.武汉大学人民医院，a.精神科；b.神经科，湖北武汉市430060。
  • 收稿日期:2013-07-08 修回日期:2013-08-19 出版日期:2013-10-25 发布日期:2013-10-25
  • 通讯作者: 王高华

Effect of Huperzine A on Spatial Memory in Rats with Repeated Electroconvulsive Stimulation

CHENG Jing, XIAO Ling, WANG Gao-hua, et al.   

  1. Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China
  • Received:2013-07-08 Revised:2013-08-19 Published:2013-10-25 Online:2013-10-25

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摘要： 目的探讨慢性重复电痉挛刺激(ECS)对大鼠空间记忆的影响，并初步研究石杉碱甲的干预作用。方法将健康成年雄性Sprague-Dawley 大鼠48 只随机分为假电痉挛刺激组(C 组)和电痉挛刺激组(E 组)，每组再分别随机分为生理盐水对照(CS 组、ES 组)和石杉碱甲(CH 组、EH组)，每组12 只。第1~17 天行生理盐水或石杉碱甲灌胃；第8~17 天给予假电痉挛刺激或电痉挛刺激；第18 天行水迷宫定位航线实验；然后各组大鼠随机取6 只于48 h 后进行空间位置探寻实验。结果CS组与CH组潜伏期无显著性差异(P>0.05)；ES组潜伏期明显长于CS组(P<0.01)；EH组潜伏期与CH组无显著性差异(P>0.05)；EH组潜伏期短于ES组(P<0.05)。CS组、CH组、EH组在隐匿平台周围区域/相反区域的搜寻时间均有显著性差异(P<0.05)。ES组在隐匿平台周围区域/相反区域的搜寻时间无显著性差异(P>0.05)。结论重复电痉挛刺激导致大鼠显著空间记忆障碍，石杉碱甲能减轻电痉挛刺激对大鼠记忆的损害。

关键词: 电痉挛刺激, 石杉碱甲, 记忆, 大

Abstract: Objective To explore the effects of Huperzine A on memory in rats with repeated electroconvulsive stimulation. Methods 48 adult male Sprague-Dawley rats were randomly divided into true electroconvulsive stimulation group (E group) and sham electroconvulsive stimulation group (C group). Then both groups were randomized to receive Huperzine A (EH group and CH group) or normal saline (ES group and CS group). There were 12 rats in each subgroup. Huperzine A or normal saline was administered during 1~17 d. During 8~17 d,true or sham electroconvulsive stimulation were administered. On the 18th d, the rats were tested in Morris water maze. Then 6 rats of each group were randomized to be taken for probe tests after 48 h. Memory test indexes include swimming speed, latency, and search time. Results There was no significant difference in the latency between CS group and CH group (P>0.05). The latency was significantly longer in ES group than in CS group (P<0.01), and shorter in EH group than in ES group (P<0.05). There were significant difference in the search time between around the hidden platform and in the opposite area in CS group, CH group and EH group (P<0.05). But there was no difference in ES group (P>0.05). Conclusion Chronic repetitive electroconvulsive stimulation can lead to spatial memory impairment in rats. Huperzine A may attenuate electroconvulsive stimulation induced amnesia.

Key words: electroconvulsive stimulation, Huperzine A, memory, rats