《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (11): 1016-1019.

• 论文 • 上一篇    下一篇

小强度跑台运动对小鼠空间学习记忆及海马糖原合成酶激酶-3β的影响

刘慧莉，赵刚   

  1. 中国医科大学运动医学系，辽宁沈阳市110001。
  • 收稿日期:2013-04-08 修回日期:2013-04-24 出版日期:2013-11-25 发布日期:2013-11-25

Effects of Low Intensity Treadmill Training on Spatial Learning and Memory and Expression of Glycogen Synthase Kinase-3β in Hippocampus in Mice

LIU Hui-li, ZHAO Gang.
   

  1. Department of Sport Medicine, China Medical University, Shenyang 110001, Liaoning,China
  • Received:2013-04-08 Revised:2013-04-24 Published:2013-11-25 Online:2013-11-25

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摘要： 目的观察小强度跑台运动对小鼠空间学习记忆能力及海马糖原合成酶激酶-3β (GSK-3β)蛋白及基因表达的影响。方法3 月龄雌性C57BL/6J 小鼠24 只，分为运动组(n=12)与对照组(n=12)。5 个月后行Morris 水迷宫测试及GSK-3β检测。结果运动组小鼠逃避潜伏期短于对照组(P<0.05)，寻找平台路径长度短于对照组(P<0.05)，穿环次数多于对照组(P<0.05)；运动组小鼠GSK-3β mRNA表达水平低于对照组(P<0.05)，p-GSK-3β-Ser9/GSK-3β比值高于对照组(P<0.05)。结论小强度跑台运动可提高小鼠空间学习和记忆能力，降低GSK-3β活性可能是其健脑作用的分子机制之一。

关键词: 跑台运动, 空间学习记忆功能, 糖原合成酶激酶-3β, 小鼠

Abstract: Objective To investigate the effects of low intensity treadmill training on spatial learning and memory and expression of glycogen synthase kinase-3β (GSK-3β) in hippocampus in mice. Methods 24 female C57BL/6J mice of 3 months were assigned into control group (n=12) and exercise group (n=12). They were assessed with Morris Water Maze task 5 months after exercise. The GSK-3β protein and mRNA expressed in hippocampus were determined 1 week after the task. Results The latency and path length to escape onto the hidden platform decreased in the exercise group (P<0.05), while the cross times increased (P<0.05) compared with the control group. The level of GSK-3β mRNA decreased (P<0.05) and ratio of p-GSK-3β-Ser9 to GSK-3β increased (P<0.05) as well. Conclusion Low intensity treadmill exercise may improve the spatial learning and memory in mice, which may down-regulate the expression and activity of GSK-3β.

Key words: treadmill exercise, spatial learning and memory, glycogen synthase kinase-3β, mice