《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (12): 1120-1123.

• 论文 • 上一篇    下一篇

针刺对高脂血症小鼠血脂的影响及其机制研究

郭佳，姬晓兰，彭博，李富运，张莉，刘承，霍则军   

  1. 1. 北京大学第三医院，北京市100191；2. 北京中医药大学，北京市100029。
  • 收稿日期:2013-09-22 修回日期:2013-11-05 出版日期:2013-12-25 发布日期:2013-12-25
  • 通讯作者: 霍则军

Effects of Acupuncture on Blood Lipids in Hyperlipidemia Mice

GUO Jia, JI Xiao-lan, PENG Bo, et al.   

  1. Peking University Third Hospital,Beijing 100191, China
  • Received:2013-09-22 Revised:2013-11-05 Published:2013-12-25 Online:2013-12-25

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被引次数

16

摘要： 目的观察针刺对高脂血症小鼠血脂的影响，探讨针刺逆转内皮功能障碍的机制。方法40 只ApoE(-/-)小鼠随机分为对照组、针刺非穴位组、针刺穴位组和他汀类药物组，分别针刺穴位、非穴位以及辛伐他汀灌胃。8 周后测量各组小鼠总胆固醇(TC)。采用ELISA 法检测血浆中血管紧张素Ⅱ(AngⅡ)、内皮素-1(ET-1)及一氧化氮(NO)的含量。采用Western blotting 法检测小鼠心脏AngⅡ 1 型受体(AT1R)、内皮素-1A受体(ETAR)的蛋白含量。结果针刺穴位组和他汀类药物组与对照组相比，TC含量降低，血浆AngⅡ、ET-1 含量降低，NO含量升高，心脏组织中AT1R、ETAR蛋白含量降低(P<0.05)。结论针刺内关和丰隆穴可以抑制ApoE(-/-)小鼠血脂升高，降低外周血AngⅡ、ET-1 水平，升高外周血NO含量，抑制心脏组织中AT1R、ETAR。

关键词: 高脂血症, 针刺, 血管紧张素Ⅱ, 内皮素-1, 受体, 小鼠

Abstract: Objective To observe the effects of acupuncture on blood lipids of hyperlipidemia mice, and explore the mechanism of acupuncture against endothelial dysfunction. Methods 40 ApoE (-/- ) mice were randomly divided into control group (C), acupuncture at non-acupoint group (D), acupuncture at acupoint group (E), and simvastatin group (F) with 10 cases in each group. After 8 weeks, total cholesterol (TC), the plasma angiotensin II (Ang II), endothelin-1 (ET-1) and nitric oxide (NO) were measured with enzyme-linked immuno sorbent assay (ELISA). The mice myocardial angiotensin II type 1 receptor (AT1R), and endothelin-1 type A receptor (ETAR) protein were detected with Western blotting. Results The blood lipid, the content of plasma Ang II and ET-1, and the level of AT1R and ETAR in the heart tissue were significantly lower, and the content of NO was significantly higher in groups E and F than in group C (P<0.05). Conclusion Acupuncture can inhibit the level of blood lipids in ApoE(-/-) mice, reduce the levels of Ang II and ET-1, increase the level of NO in peripheral blood, and inhibit the expression of AT1R, ETAR in heart tissue.

Key words: hyperlipidemia, acupuncture, angiotensin II, endothelin-1, receptor, mice