《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (2): 104-106.

• 论文 • 上一篇    下一篇

莫诺苷对局灶性脑缺血再灌注大鼠凝血四项的影响

袁廷伟，孙芳玲，王晓峰，艾厚喜，王文，龚大春   

  1. 1.三峡大学，湖北宜昌市443002；2.首都医科大学宣武医院药物研究室，北京市100053。
  • 收稿日期:2012-11-22 修回日期:2013-01-09 出版日期:2013-02-25 发布日期:2013-02-25
  • 通讯作者: 龚大春

Effect of Morroniside on Coagulation in Focal Cerebral Ischemia/Reperfusion in Rats

YUAN Ting-wei, SUN Fang-ling, WANG Xiao-feng, et al.   

  1. China Three-Gorges University, Yichang 443002, Hubei, China
  • Received:2012-11-22 Revised:2013-01-09 Published:2013-02-25 Online:2013-02-25

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摘要： 目的研究莫诺苷对局灶性脑缺血再灌注大鼠纤维蛋白原(Fib)、凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)和凝血酶时间(TT)的影响。方法用线栓法制备大鼠局灶性脑缺血再灌注模型，造模后，分别给予莫诺苷低剂量(30 mg/kg)、中剂量(90 mg/kg)、高剂量(270 mg/kg)连续灌胃7 d，阿司匹林(ASA)为阳性对照药。使用试剂盒分别检测Fib、PT、APTT、TT指标的变化。结果与假手术组相比，模型组Fib 含量显著增加(P<0.001)，PT、APTT、TT显著缩短(P<0.001)；与模型组相比，莫诺苷能明显降低Fib含量(P<0.01)，延长PT、APTT、TT(P<0.05)。结论莫诺苷具有对抗局灶性脑缺血再灌注大鼠凝血功能的作用。

关键词: 莫诺苷, 凝血, 缺血/再灌注, 大鼠

Abstract: Objective To investigate the effects of morroniside on fibrinogen (Fib), prothrombin time (PT), activated partial thromboplastin time (APTT) and thrombin time (TT) in focal cerebral ischemia/reperfusion in rats. Methods The animal model was induced with occlusion of middle cerebral artery (MCAO) with suture embolus, and morroniside was then administered intragastrically at the dose of 30, 90 and 270 mg/kg for 7 d. Acetyl salicylic acid was taken as positive control drug. The content of Fib, and PT, APTT and TT were measured withrelated kits. Results Compared with the sham-operated group, the concentration of Fib significantly increased (P<0.001) and PT, APTT and TT significantly shortened in the model group (P<0.001); however, compared with the model group, morroniside significantly decreased Fib content (P<0.01) and prolonged PT, APTT and TT (P<0.05). Conclusion Morroniside can antagonize the coagulation function in focal cerebral ischemia/reperfusion in rats.

Key words: morroniside, coagulation, ischemia/reperfusion, rats