《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (2): 119-123.

• 论文 • 上一篇    下一篇

电针对局灶性脑缺血再灌注大鼠神经生长相关蛋白-43 和Nogo-A表达的影响

韩永升，韩咏竹，徐磊，刘向国，徐银   

  1. 1.安徽中医学院神经病学研究所附属医院，安徽合肥市230061；2.蚌埠医学院第一附属医院康复医学科，安徽蚌埠市233004；3.安徽中医学院中西医结合临床学院，安徽合肥市230038。
  • 收稿日期:2012-09-08 修回日期:2012-10-24 出版日期:2013-02-25 发布日期:2013-02-25

Effects of Electroacupuncture on Expression of Growth Associated Protein-43 and Nogo-A after Focal Cerebral Ischemia/Reperfusion in Rats

HAN Yong-sheng, HAN Yong-zhu, XU Lei, et al.   

  1. Institute of Neurology, Anhui Traditional Chinese Medical College, Hefei 230061, Anhui, China
  • Received:2012-09-08 Revised:2012-10-24 Published:2013-02-25 Online:2013-02-25

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摘要： 目的探讨电针对局灶性脑缺血再灌注模型大鼠神经功能恢复和脑组织神经生长相关蛋白-43(GAP-43)、Nogo-A表达的影响。方法48 只健康成年雄性Sprague-Dawley 大鼠分为假手术组、模型组和电针组，每组16 只。采用线栓法制作大鼠大脑中动脉缺血再灌注模型。电针组于造模成功90 min 后电针刺双侧内关、水沟、三阴交和百会30 min，每天1 次。假手术组和模型组不进行任何干预治疗。各组大鼠在造模后7 d、14 d 各取8 只进行神经功能评估及免疫组化SP法观察脑组织GAP-43、Nogo-A的表达。结果假手术组大鼠无神经功能缺损症状；7 d 时，模型组和电针组神经功能评分无显著性差异(P>0.05)，14 d 时，电针组神经功能优于模型组(P<0.05)。假手术组各时间点未见GAP-43 阳性细胞表达。7 d 时，模型组缺血灶周围脑组织出现GAP-43 阳性细胞，14 d 时减少；电针组GAP-43 阳性细胞各时间点较模型组明显增加(P<0.01)。假手术组仅见少量Nogo-A表达，模型组脑缺血区7 d、14 d 时Nogo-A表达明显增多(P<0.01)，电针组Nogo-A表达在各时间点较模型组明显减少(P<0.01)。结论电针能通过促进局灶性脑梗死大鼠脑组织内GAP-43 表达、抑制Nogo-A表达，改善大鼠的神经功能。

关键词: 缺血/再灌注, 电针, 神经功能, 神经生长相关蛋白-43, Nogo-A, 大鼠

Abstract: Objective To investigate the effect of electroacupuncture on expression of growth associated protein-43 (GAP-43) and Nogo-A in brain after focal cerebral ischemia/reperfusion in rats. Methods 48 male Sprague-Dawley rats were randomly divided into sham group, model group and electroacupuncture group, and the latter two were modeled as middle cerebral artery occlusion and reperfusion with nylon monofilament suture. Electroacupuncture was performed 90 min after modeling in the electroacupucture group at acupoints of Neiguan (PC06), Shuigou (DU26), Sanyinjiao (SP06), Baihui (DU20), for 30 min, once a day. The sham group and the model group were conventionally fed in cages without any intervention. 8 rats of each group were assessed with Longa's score, and the expressions of GAP-43 and Nogo-A were detected with immunohistochemistry 7 d and 14 d after modeling respectively. Results The sham group presented no neurological symptoms. There was not different in Longa's score between the model group and the electroacupuncture group 7 d after modeling (P>0.05), but was different 14 d after modeling (P<0.05). GAP-43 positive cells was not found in the sham group, but could be found around cerebral ischemia 7 d after modeling, which decreased 14 d after modeling in the model group. GAP-43 positive cells increased significantly in the electroacupuncture group compared with the model group at each time (P<0.01). Nogo-A positive cells was few found in the sham group, and was more in the model group (P<0.01). The expression of Nogo-A decreased significantly in the electroacupuncture group compared with the model group at each time (P<0.01). Conclusion Electroacupuncture can improve neurological function of focal cerebral ischemia/reperfusion rats, which may be associated with the increase of GAP-43 and descrease of Nogo-A in peri-infarct regions

Key words: ischemia/reperfusion, electroacupuncture, neurological function, growth associated protein-43, Nogo-A, rats