《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (2): 124-128.

• 论文 • 上一篇    下一篇

低声压级次声对大鼠脑缺血再灌注后胶质纤维酸性蛋白表达的影响

李德洁，范建中，吴红瑛，何任红，陈琦，刘夏   

  1. 南方医科大学南方医院康复医学科，广东广州市510515。
  • 收稿日期:2012-08-13 修回日期:2012-09-17 出版日期:2013-02-25 发布日期:2013-02-25
  • 通讯作者: 范建中

Effect of Infrasound on Expression of Glial Fibrillary Acidic Protein after Cerebral Ischemia/Reperfusion in Rats

LI De-jie, FAN Jian-zhong, WU Hong-ying, et al   

  1. Department of Rehabilitation, Nanfang Hospital of Southern Medical University, Guangzhou 510515,Guangdong, China
  • Received:2012-08-13 Revised:2012-09-17 Published:2013-02-25 Online:2013-02-25

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摘要： 目的探讨低声压级次声对大鼠脑缺血再灌注后周围胶质纤维酸性蛋白(GFAP)表达的影响。方法线栓法制作大脑中动脉脑缺血(MCAO)2 h 再灌注模型。将36 只雄性Sprague-Dawley 大鼠随机分成假手术组(n=12)、次声组(n=12)和模型组(n=12)，每组再分为3 d、7 d 两个亚组，每组6 只。次声组在缺血再灌注12 h 后连续予次声干预2 h/d，模型组干预过程中除不开机外，其余过程同次声组，假手术组不做任何干预。在治疗3 d、7 d 后(处死前)对大鼠进行神经功能缺损评分(mNSS)，脑组织切片采用免疫组织化学方法观察脑缺血周围GFAP表达变化。结果次声7 d 组大鼠mNSS评分较模型组降低(P<0.05)；次声组脑缺血周围GFAP表达的积分光密度(IOD)均较模型组显著增高(P<0.001)。结论低声压级次声能增强大鼠脑缺血再灌注后GFAP的表达。

关键词: 次声, 缺血/再灌注, 胶质纤维酸性蛋白

Abstract: Objective To explore the effect of infrasound on the expression of glial fibrillary acidic protein (GFAP) after cerebral ischemia/reperfusion in rats. Methods The model of cerebral ischemia/reperfusion in rats was induced with intraluminal middle cerebral artery occlusion (MCAO) with nylon monofilament suture. 36 male adult Sprague-Dawley rats were randomly divided into sham group (n=12),model group (n=12) and infrasound group (n=12), then each group was randomly divided into 3 d and 7 d subgroups, with 6 rats in each subgroup.The infrasound group was treated with infrasound for 2 h every day 12 h after operation, the model group was treated in the same way turning off the power, the sham group received no treatment. They were evaluated with the modified Neurological Severity Score (mNSS) 3 and 7 d after treatment (before being executed), and brain tissue slices were immunohistochemically stained to observe the expression of GFAP around the ischemic sites. Results Compared to the model group, the mNSS score in 7 d infrasound group decreased significantly (P<0.05), the integral optical density (IOD) of GFAP around the focus was significantly higher in the infrasound group than in the model group (P<0.001). Conclusion Infrasound can increase the expression of GFAP after cerebral ischemia/reperfusion in rats.

Key words: infrasound, ischemia/reperfusion, glial fibrillary acidic protein