《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (4): 334-340.

• 基础研究 • 上一篇    下一篇

电针阿是穴对骨骼肌损伤大鼠肌肉再生及碱性成纤维细胞生长因子表达的影响

陈欢1，张莉1，崔强2，卢虎英3，卫肖艳1   

  1. 1.北京中医药大学针灸推拿学院，北京市 100029；2.陕西中医医院，陕西西安市 710003；3.中国康复研究中心北京博爱医院，北京市 100068
  • 收稿日期:2012-10-29 修回日期:1900-01-01 出版日期:2013-04-25 发布日期:2013-04-25

Effects of Electroacupuncture at Ouch Point on Muscle Regeneration and Basic Fibroblast Growth Factor Expression in Skeletal Muscle Damaged Rats

CHEN Huan, ZHANG Li, CUI Qiang, et al.   

  1. School of Acupuncture, Moxibustion and Tuina, Beijing University ofChinese Medicine, Beijing 100029, China
  • Received:2012-10-29 Revised:1900-01-01 Published:2013-04-25 Online:2013-04-25

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摘要： 目的　观察电针阿是穴对骨骼肌损伤大鼠腓肠肌增殖细胞核抗原(PCNA)、结蛋白(Desmin)及碱性成纤维细胞生长因子(bFGF)表达的影响，探讨电针阿是穴对骨骼肌损伤后肌卫星细胞增殖分化的可能机制。方法　将78 只雄性Sprague-Dawley 大鼠随机分为模型组6 只，空白组、电针组、自然修复组各24 只，每组再分为1 d、4 d、7 d、14 d 4 个亚组，各6 只。模型组、自然修复组与电针组采用腓肠肌钝挫伤结合离心运动的方法造模，模型建立后，模型组取腓肠肌行HE染色，其余各组按时间点相应处理并取腓肠肌行PCNA、Desmin 和bFGF免疫组化染色。结果　自然修复组各时间点PCNA表达均明显高于空白组(P<0.01)；Desmin在损伤后1 d、14 d 低于空白组(P<0.05)，损伤后7 d 高于空白组(P>0.05)；bFGF 在损伤后7 d 高于空白组(P<0.01)。电针组PCNA、bFGF 与Desmin 的表达在损伤后1 d、4 d 均高于自然修复组(P<0.05)。结论　电针阿是穴能促进肌卫星细胞增殖，加快其成肌分化，缩短损伤修复进程，其机制可能与上调及提前bFGF的表达有关。

关键词: 骨骼肌损伤, 电针, 阿是穴, 增殖细胞核抗原, 碱性成纤维细胞生长因子, 结蛋白, 大鼠

Abstract: Objective To observe the effects of electroacupuncture at Ouch Point on proliferation cell nuclear antigen (PCNA), Desmin and basic fibroblast growth factor (bFGF) expression in gastrocnemius muscle, and explore the possible mechanisms of the effect. Methods 78 male Sprague-Dawley rats were randomly divided into model group (n=6), blank group (n=24), self-repair group (n=24) and electroacupuncture group (n=24). Each of the later 3 groups was divided into 1 d, 4 d, 7 d and 14 d subgroups, 6 rats in each subgroup. The model group, self-repair group and electroacupuncture group were modeled by gastrocnemius muscle crush injury combined with eccentric exercise-induced injury. After that, the model group given HE stain, and the other groups were given correspondent approach according to time point and immunohistochemical technology was used to test the expression of PCNA, Desmin and bFGF. Results PCNA expression was higher in the self-repair group than in the blank group at each time point (P<0.01). Desmin expression was lower in the self-repair group than in the blank group 1 d and 14 d after injury (P<0.05), but higher 7 d after injury (P>0.05). bFGF expression was higher in the self-repair group than in the blank group 7 d after injury (P<0.01). PCNA, bFGF and Desmin expression were higher in the electroacupuncture group than in the self-repair group 1 d and 4 d after injury (P<0.05). Conclusion Electroacupuncture at Ouch Point can promote the proliferation of muscle satellite cells, accelerate the myogenic differentiation and shorten the repair process, which may be related to the increased and advanced expression of bFGF.

Key words: skeletal muscle damage, electroacupuncture, Ouch Point, proliferating cell nuclear antigen, basic fibroblast growth factor, Desmin, rats