《中国康复理论与实践》

《中国康复理论与实践》 ›› 2014, Vol. 20 ›› Issue (1): 34-36.

• 论文 • 上一篇    下一篇

神经节苷脂-1 与康复训练对缺血缺氧性脑损伤大鼠神经功能的影响

王珂，杨会娟，马俊，候梅   

  1. 1.青岛市妇女儿童医院，山东青岛市266034；2.莒县人民医院儿保科，山东莒县276500。
  • 收稿日期:2013-05-06 修回日期:2013-07-08 出版日期:2014-01-25 发布日期:2014-01-25
  • 通讯作者: 候梅

Effect of Ganglioside- 1 and Rehabilitation on Recovery of Neurologic Behavior in Rats with Hypoxic- ischemic Brain Damage

WANG Ke, YANG Hui- juan, MA Jun, et al.   

  1. Department of Neurological Rehabilitation, Qingdao Women ＆ Children Hospital, Qingdao 266034, Shandong, China
  • Received:2013-05-06 Revised:2013-07-08 Published:2014-01-25 Online:2014-01-25

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摘要： 目的探讨神经节苷脂-1(GM1)和康复训练对大鼠缺血缺氧性脑损伤(HIBD)后功能恢复的影响及可能机制。方法48只Sprague-Dawley 幼鼠建立HIBD模型，分为对照组、GM1 组、康复训练(R)组、GM1+康复训练(GM1+R)组及假手术组。各组大鼠采用悬吊试验、斜坡试验和水迷宫试验进行评定。免疫组化法检测海马、额叶皮质神经元活化型caspase-3 表达。结果各组间悬吊试验、斜坡试验结果有非常高度显著性差异(P<0.001)，从高到低依次为假手术组、GM1+R组、R组、GM1 组和对照组(P<0.05)。各组间寻找平台潜伏期有非常显著性差异(P<0.01)，对照组和GM1 组较假手术组长(P<0.05)。GM1 组和R组的海马、额叶皮质活化型caspase-3 水平与假手术组有显著性差异(P<0.05)，GM1 组、R组及GM1+R组海马活化型caspase-3 与对照组有显著性差异(P<0.05)，R组和GM1+R组额叶皮质活化型caspase-3 与对照组有显著性差异(P<0.05)。海马、额皮质活化型caspase-3 的表达与悬吊试验、斜坡试验结果正相关(P<0.05)，与水迷宫试验无明显相关(P>0.05)。结论早期康复训练及应用GM1 对HIBD神经功能恢复有一定疗效，联合应用存在协同效应。其机制可能与神经元末梢突触后存在大量活化型caspase-3 有关。

关键词: 缺血缺氧性脑损伤, 神经节苷脂-1, 康复, caspase-3, 大鼠

Abstract: Objective To investigate the effects and the possible mechanism of Ganglioside-1 (GM1) and rehabilitation on neurologic behaviors of rats with hypoxic-ischemic brain damage (HIBD). Methods 48 immature rats developed as model of HIBD were divided into 5 groups: control group, GM1 group, rehabilitation training group (R group), GM1+R group, and sham group. They were evaluated with Climb, Slope and Water Maze Tests. The expressions of activated caspase-3 in neurons were detected with immunohistochemistry. Results The achievement of Climb and Slope Test were significantly different among groups (P<0.001), which ranked as the sham group> GM1+R group> R group> GM1 group> control group (P<0.05). The latency of Water Maze Test was significantly different among groups (P<0.01),which was longer in the control and GM1 groups than in the sham group (P<0.05). The activated caspase-3 expressed in hippocampus and frontal cortex was less in the GM1 and R groups than in the sham group (P<0.05), more in the GM1 group, R group and GM1+R group than in the control group in hippocampus (P<0.05), more in the R group and GM1+R group than in the control group in frontal cortex (P<0.05).The expression of activated caspase-3 in both hippocampus and frontal cortex correlated with the achievement of Climb and Slope Test (P<0.05), but not with the latency of Water Maze Test (P>0.05). Conclusion Rehabilitation training and GM1 are effective on recovery of neural function in rats with HIBD with synergia, which may associated with the activated caspase-3 in terminal synapse.

Key words: hypoxic-ischemic brain damage, ganglioside-1, rehabilitation, caspase-3, rats