《中国康复理论与实践》

《中国康复理论与实践》 ›› 2014, Vol. 20 ›› Issue (12): 1122-1125.

• 础基研究 • 上一篇    下一篇

骨髓基质细胞植入对脑缺血大鼠海马齿状回突触形成及记忆能力的影响

罗开源1，钟小明1，余鸿2
   

  1. 1.江西省赣南医学院第一附属医院儿科，江西赣州市341000；2.四川省泸州医学院组织与胚胎学教研室，四川泸州市646000。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-12-25 发布日期:2014-12-25

Effect of Bone Marrow Stromal Cells Transplantation on Synapse Formation in Dentate Gyrus and Memory in Rats with Ischemic Brain Injury

LUO Kai-yuan, ZHONG Xiao-ming, YU Hong.
  

  1. Department of Pediatrics, The First Affiliated Hospital of Gannan Medical College, Ganzhou, Jiangxi 341000, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-12-25 Online:2014-12-25

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摘要： 目的探讨骨髓基质细胞(BMSCs)经侧脑室植入脑缺血大鼠后海马齿状回突触形成及大鼠记忆能力的变化。方法大鼠BMSCs培养3 代。60 只新生Sprague-Dawley大鼠分为假手术组、模型组、细胞组各20 只，后两组结扎左侧颈总动脉，细胞组于7 d 后经侧脑室植入BMSCs。8 周后行Morris 水迷宫测试。测试后取脑组织，免疫组织化学染色检测海马齿状回处突触素表达。结果模型组探查训练(T1)和对位探查训练(T2)时间均较假手术组缩短(P<0.05)；细胞组T1 和T2 分别较模型组延长(P<0.05)。模型组大鼠海马突触素阳性细胞积分光密度值(IOD)较假手术组减小(P<0.05)，细胞组IOD较模型组增加(P<0.05)。结论BMSCs经侧脑室植入可促进脑缺血大鼠海马齿状回突触形成，并改善其记忆功能。

关键词: 骨髓基质细胞, 移植, 缺血, 脑损伤, 突触, 记忆

Abstract: Objective To observe the synapse formation in hippocampal dentate gyrus, and the memory ability after transplanting bone marrow stromal cells (BMSCs) into lateral ventricle of ischemic brain injury rats. Methods BMSCs from femur of a Sprague-Dawley rat were cultured for 3 generations in vitro. 60 newborn Sprague-Dawley rats were divided into sham group (n=20), model group (n=20) and BMSCs group (n=20). The latter 2 groups were ligated the left common carotid artery, and the BMSCs group were injected BMSCs into the lateral ventricle 7 days after ligation. They were tested with Morris's water maze 8 weeks later, and then, the brains were immunohistochemical staining to detect synaptophysin (SY). Results The time of probe trial of acquisition phase (T1) and reveral phase (T2) decreased in the model group compared with the sham group (P<0.05), and increased in the BMSCs group compared with the model group (P<0.05). The integral optical density (IOD) of SY positive cells decreased in the model group compared with the sham group (P<0.05), and increased in the BMSCs group compared with the model group (P<0.05). Conclusion BMSCs implantation through lateral ventricle can improve the learning and memory ability of rats, which may associated with the synapse formation in dentate gyrus.

Key words: bone marrow stromal cells, transplantation, ischemia, brain injury, synapse, memory