《中国康复理论与实践》

《中国康复理论与实践》 ›› 2014, Vol. 20 ›› Issue (2): 121-124.

• 论文 • 上一篇    下一篇

体外冲击波对兔膝骨关节炎软骨白细胞介素-1β及肿瘤坏死因子-α表达的影响

侯晓东，刘洪柏，刘克敏   

  1. 1.广东省人民医院，广东省医学科学院，广东广州市528251；2.中国康复研究中心北京博爱医院骨科，北京市100068。
  • 收稿日期:2013-02-28 修回日期:2013-10-08 出版日期:2014-02-25 发布日期:2014-02-25
  • 通讯作者: 刘克敏

Effect of Extracorporeal Shock Wave on Expression of Interleukin-1β and Tumour Necrosis Factor-α in Cartilage in Rabbits with Knee Osteoarthritis

HOU Xiao-dong, LIU Hong-bo, LIU Ke-min.   

  1. Guangdong People's Hospital, Guangdong Academy of Medical Sciences,Guangzhou 528251, Guangdong, China
  • Received:2013-02-28 Revised:2013-10-08 Published:2014-02-25 Online:2014-02-25

PDF (PC)

被引次数

23

摘要： 目的观察体外冲击波(ESW)对兔膝骨性关节炎软骨白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)水平的影响。方法将30 只新西兰大白兔随机分为对照组、模型组和治疗组，每组各10 只。模型组和治疗组采用改良伸直位固定6 周，制备兔膝骨关节炎模型。治疗组造模后立即给予ESW治疗1 次，能流密度0.1 mJ/mm2，冲击次数1000 次。各组分别于治疗后4 周处死，观察大体标本形态学变化，采用免疫组织化学法检测各组关节软骨中IL-1β和TNF-α的水平。结果治疗组IL-1β 和TNF-α的表达较模型组明显下调(P<0.01)。结论ESW能下调兔膝骨关节炎软骨细胞IL-1β、TNF-α的表达。

关键词: 骨关节炎, 体外冲击波, 关节软骨, 白细胞介素-1β, 肿瘤坏死因子-α, 兔

Abstract: Objective To explore the effect of extracorporeal shock wave (ESW) on expression of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in cartilage in rabbits with knee osteoarthritis. Methods 30 rabbits were randomly divided into control group, model group and treatment group, with 10 rabbits in each group. Model of knee osteoarthritis was established with modified plaster cast in extension position for 6 weeks except the control group, and the treatment group was treated with ESW (each 1000 impulse, the energy flux density of 0.1 mJ/mm2). The rabbits were sacrificed respectively 4 weeks after ESW treatment, the general and histological changes of articular cartilage were examined and immunohistochemical staining was used to observe the expression of IL-1β and TNF-α in cartilage. Results The expression of IL-1β and TNF-α were significantly lower in the treatment group than in the model group (P<0.01). Conclusion ESW can down-regulate the expression of IL-1β and TNF-α in chondrocytes with osteoarthritis.

Key words: osteoarthritis, extracoporeal shock wave, articular cartilage, interleukin-1β, tumour necrosis factor-α, rabbits